Wnt/β-Catenin Activated Targets Antibody Sampler Kit #8655
Product Information
Kit Usage Information
Protocols
- 2203: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow, ChIP Magnetic
- 2230: Western Blotting, Immunofluorescence, Flow
- 2978: Western Blotting, Immunohistochemistry (Paraffin)
- 3570: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, Flow*
- 3801: Western Blotting, Immunohistochemistry (Paraffin)
- 5605: Western Blotting, Immunofluorescence
- 7074: Western Blotting
- 7076: Western Blotting
- 8198: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 9165: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow Triton Permeabilization (Rabbit), ChIP Magnetic, Chromatin IP-seq
Product Description
The Wnt/β-Catenin Activated Targets Antibody Sampler Kit provides an economical means to investigate target proteins of the Wnt/β-Catenin signaling pathway. This kit contains enough primary antibody to perform two western blots per primary.
Specificity / Sensitivity
Each antibody in the Wnt/β-Catenin Activated Targets Antibody Sampler Kit detects its respective target at endogenous levels. LEF1 (C12A5) Rabbit mAb does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. c-Myc (D84C12) Rabbit mAb is not recommended for detection of Myc-tagged fusion proteins. TCF1/TCF7 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1/TCF7 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1.
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing animals with a GST-c-Jun protein corresponding to the amino-terminal sequence of human c-Jun protein or synthetic peptides corresponding to residues near the amino terminus of c-Myc protein, residues surrounding Pro82 of human LEF1 protein, residues surrounding Ile264 of mouse MMP-7 protein, residues surrounding Pro95 of human TCF1/TCF7 protein, residues near the carboxy terminus of human Met protein, or residues near the carboxy-terminus of human cyclin D1 protein.
The mouse monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes for CD44.
The mouse monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes for CD44.
Background
The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11).
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- Wilkins, J.A. and Sansom, O.J. (2008) Cancer Res 68, 4963-6.
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- Brabletz, T. et al. (1999) Am J Pathol 155, 1033-8.
- Clevers, H. and Nusse, R. (2012) Cell 149, 1192-205.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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