SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded head and neck carcinoma using TIM-3 (D5D5R™) & CO-00010-488 SignalStar™ Oligo-Antibody Pair #81365 (green), PD-1 (Intracellular Domain) (D4W2J) & CO-0008-594 SignalStar™ Oligo-Antibody Pair #35347 (yellow), PD-L1 (E1L3N®) & CO-0005-594 SignalStar™ Oligo-Antibody Pair #28249 (red), LAG3 (D2G4O™) & CO-0026-750 SignalStar™ Oligo-Antibody Pair #85452 (cyan), CD3ε (D7A6E™) & CO-0001-488 SignalStar™ Oligo-Antibody Pair #92856 (pink), CD68 (D4B9C) & CO-0007-594 SignalStar™ Oligo-Antibody Pair #77318 (orange), CD8α (D8A8Y) & CO-0004-647 SignalStar™ Oligo-Antibody Pair #66676 (magenta),Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (grey), CD206/MRC1 (E6T5J) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #59414 (teal), CD20 (E7B7T) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #83399 (lavender), CD45 (Intracellular Domain) (D9M8I) XP® Rabbit mAb (Alexa Fluor® 750 Conjugate) (olive), and DAPI #4083 (blue). 所有荧光团都被分配了伪彩，如图所示。在 Leica Biosystems 的 BOND RX 自动染色机上进行染色。
Context matters. Spatial biology uses techniques such as multiplex immunohistochemistry (mIHC) to identify which cells are present and how they behave within their tissue environment. Multiplexing 2-12 targets at a time provides a full picture on how and where critical biological events occur, helping researchers understand disease progression and identify biomarker co-expression.
CST offers a diverse portfolio of easy-to-use, flexible, and validated multiplex assay solutions that are specific to the number of targets you want to monitor. Performing a 2-plex or 3-plex experiment? IHC-validated direct conjugate antibodies will deliver reliable results you trust. Need 3-plex to 8-plex capabilities? SignalStar™ mIHC is the solution for you. Want to get the most out of your tissue? You’ll be able to multiplex 8+ targets using a combination of SignalStar technology, IHC-validated direct conjugates, and the fluorescence removal kit.
We have organized a collection of mIHC resources, organized by the plex that meets your needs, to help you get data you need while maintaining spatial context and tissue integrity.