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Oligodendrocyte Marker Antibody Sampler Kit #38289

Oligodendrocyte Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various tissues using PLP1 (E9V1N) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The upper band corresponds to the full-length PLP1 while the lower band is an alternative splice from PLP1 known as DM20.

To Purchase # 38289

  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
Olig2 (E6G6Q) XP® Rabbit mAb #6591520 µlWB, IP, IHC, IFH M R35Rabbit IgG
Myelin Basic Protein (D8X4Q) XP® Rabbit mAb #7889620 µlWB, IHC, IFH M R12-18Rabbit IgG
CNPase (D83E10) XP® Rabbit mAb #566420 µlWB, IP, IFH M R47Rabbit IgG
PLP1 (E9V1N) Rabbit mAb #2870220 µlWB, IHC, IFH M R20-30Rabbit IgG
MAG (D4G3) XP® Rabbit mAb #904320 µlWB, IP, IFH M R100Rabbit IgG
MOG (E5K6T) XP® Rabbit mAb #9645720 µlWB, IHCH M R46, 35, 28, 23Rabbit IgG
NG2/CSPG4 (E3B3G) XP® Rabbit mAb #4391620 µlWB, IHC, IFH250, 450Rabbit IgG
Sox10 (E6B6I) XP® Rabbit mAb #6966120 µlWB, IP, IHCH62Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBRabGoat 

Product Information

Product Description

The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot. This kit includes enough primary antibodies to perform at least twenty IF tests or two western blot experiments per primary antibody.

Background

Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7). Chondroitin sulfate proteoglycan 4 (CSP4, NG2) is a type I membrane glycoprotein expressed by oligodendroglial precursor cells (OPCs) that plays a role in cell attachment and migration (8). SRY-box 10 (Sox10) is a high-mobility group transcription factor expressed throughout oligodendrocyte development required for myelin gene expression (9,10). Myelin-oligodendrocyte glycoprotein (MOG) is a type I membrane bound glycoprotein of the immunoglobulin superfamily that is enriched in the outer lamella of the myelin sheath (11). Olig2 is a basic helix–loop–helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the CNS (12,13). Olig2 has been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (14,15).
For Research Use Only. Not For Use In Diagnostic Procedures.
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