Render Target: SSR
Render Timestamp: 2024-12-10T22:12:04.412Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-12-05 11:37:10.983
Product last modified at: 2024-12-06T10:00:07.916Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Anti-rabbit IgG, HRP-linked Antibody #7074

Filter:
  • WB

    Supporting Data

    REACTIVITY Rab
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Rab-Rabbit 

    Product Information

    Product Description

    Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.  This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.

    Product Usage Information

    Recommended Antibody Dilutions:

    1:1000–1:3000

    20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K

    SignalFireTM ECL Reagent #6883 1:1K–1:3K

    SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K

    SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K

    Storage

    Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.

    Protocol

    Specificity / Sensitivity


    Species Reactivity:

    Rabbit

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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