Render Target: SSR
Render Timestamp:
4/7/2025, 12:50:51 AM EDT
4/7/2025, 4:50:51 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:15:15.994
Product last modified at: 2025-03-19T20:15:08.280Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NG2/CSPG4 (E3B3G) XP® Rabbit mAb #43916

Filter:
  • WB
  • IHC
  • IF
Western Blotting Image 1: NG2/CSPG4 (E3B3G) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines using NG2/CSPG4 (E3B3G) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, NG2/CSPG4 protein is not expressed in MCF7 cells.

To Purchase # 43916

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 250, 450
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunohistochemistry (Paraffin) 1:100 - 1:400
Immunofluorescence (Immunocytochemistry) 1:200 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #25162.

Protocol

Specificity / Sensitivity

NG2/CSPG4 (E3B3G) XP® Rabbit mAb recognizes endogenous levels of total NG2/CSPG4 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1410 of human NG2/CSPG4 protein.

Background

The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility (1). The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42, and p130 Cas (2). PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia accompanied by enhanced cell motility (3). ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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