SMAD 1/5/9 Antibody Sampler Kit #12656
Product Information
Kit Usage Information
Protocols
- 5753: Western Blotting, Immunoprecipitation (Magnetic)
- 6944: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic
- 7074: Western Blotting
- 9516: Western Blotting, Immunofluorescence*, Flow Cytometry, BSA-free Methanol Permeabilization Protocol
- 12534: Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
- 13820: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence*, Flow
- 38454: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq
Product Description
The SMAD1/5/9 Antibody Sampler Kit provides an economical means of detecting target proteins of the BMP signaling pathway. The kit includes enough antibody to perform two western blots with each primary antibody.
Specificity / Sensitivity
Each antibody in the SMAD1/5/9 Antibody Sampler Kit recognizes endogenous levels of its respective target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site(s).
Source / Purification
Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser206 of human SMAD1 protein or Ser463/465 of human SMAD1 and SMAD5 proteins. Total SMAD1, SMAD4, and SMAD5 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser190 of human SMAD1, Asp165 of human SMAD4, or Pro249 of human SMAD5 protein.
Background
Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/9 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smads by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smads regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-7) .
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限制使用
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