Parthanatos Antibody Sampler Kit #26768
Product Information
Kit Usage Information
Protocols
- 5318: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence
- 7074: Western Blotting
- 9532: Western Blotting, Immunoprecipitation (Agarose)
- 75038: Western Blotting, Immunofluorescence, Immunofluorescence
- 89190: Western Blotting, Immunofluorescence*
Product Description
The Parthanatos Antibody Sampler Kit provides an economical means of detecting the activation of parthanatos. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Specificity / Sensitivity
Each antibody in the Parthanatos Antibody Sampler Kit detects endogenous levels of its target protein. PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP-1 and the large fragment (89 kDa) produced by caspase cleavage at Asp214. This antibody does not cross-react with PARP-2 or PARP-3. Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb recognizes endogenous levels of ADP-ribosylated proteins and does not cross-react with other post-translational modifications.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Gly623 of human PARP-1, Ala520 of human AIF, and Tyr100 of human MIF protein, or with KLH modified on lysines with ADP ribose.
Background
Parthanatos is a form of regulated cell death that follows a multistep cascade and is triggered by the accumulation of poly (ADP-ribose) (PAR). When PAR polymerase-1 (PARP-1) is overactivated under certain conditions, excessive PAR is produced and binds to apoptosis-inducing factor (AIF). As a result, AIF is released from the mitochondria and forms a complex with macrophage migration inhibitory factor (MIF). Subsequently, the AIF/MIF complex is translocated to the nucleus where MIF cleaves genomic DNA into large fragments, and cell death follows (1-3). Studies have found that parthanatos is involved in the pathogenesis of many diseases, particularly neurodegenerative disorders, such as Alzheimer's disease, Huntington's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS) (4-7).
- Fatokun, A.A. et al. (2014) Br J Pharmacol 171, 2000-16.
- Wang, Y. et al. (2016) Science 354, aad6872. doi: 10.1126/science.aad6872.
- Liu, L. et al. (2022) Cell Mol Life Sci 79, 60.
- Park, H. et al. (2020) Int Rev Cell Mol Biol 353, 1-29.
- Wang, X. and Ge, P. (2020) Neuroscience 449, 241-250.
- Zhou, Y. et al. (2021) Pharmacol Res 163, 105299.
- Thapa, K. et al. (2021) Life Sci 267, 118975.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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