Pancreatic Marker IHC Antibody Sampler Kit #28002
Product Information
Kit Usage Information
Protocols
- 3014: Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
- 4545: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 4593: Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence
- 7074: Western Blotting
- 7076: Western Blotting
- 8233: Immunohistochemistry (Paraffin), Immunofluorescence
- 23451: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin)
- 54551: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 60433: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence
Product Description
The Pancreatic Marker IHC Antibody Sampler Kit provides a useful selection of markers to distinguish pancreatic cell types that perform important functions to maintain glucose homeostasis. The kit also includes antibodies that differentiate pancreatic tumor subtypes. The sampler kit is designed for use on formalin-fixed, paraffin-embedded tissue samples.
Specificity / Sensitivity
Each antibody in the Pancreatic Marker IHC Antibody Sampler Kit detects endogenous levels of its target protein. Pan-Keratin (C11) Mouse mAb detects total keratin 4, 5, 6, 8, 10, 13, and 18. The antibody does not cross-react with other keratins. NKX6.1 (D8O4R) Rabbit mAb recognizes total NKX6.1 protein. Note: Some non-specific staining of squamous epithelium has been observed by IHC. It is unclear if the antibody works on human samples in western blotting but is predicted to based on 100% sequence homology.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to Val42 of human insulin, Ala137 of human proglucagon, residues near the carboxy terminus of human Pax6, and to human CD200 recombinant protein. Pan-Keratin (C11) Mouse mAb (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human glucagon, and the sequence of human C-peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Insulin is a hormone that is produced and released from pancreatic β cells through a glucose sensing pathway. Proinsulin is the precursor molecule to insulin and is processed prior to its secretion. Insulin is composed of A-peptide and B-peptide which are joined by a disulfide bond. The center one-third of the molecule is cleaved and released as C-peptide, which has a longer half-life than insulin (1). Antibodies to both insulin and C-peptide are useful markers for β cells.
Glucose homeostasis is regulated by a variety of hormones including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (2). Antibodies to glucagon and proglucagon are useful markers for pancreatic α cells.
NKX6.1 is an important transcription factor in a network of transcription factors that are critical for pancreatic β cell development and maintenance (3). Antibodies to NKX6.1 are useful markers for β cells.
Pan-Keratin and CD200 antibodies are useful to mark and differentiate some pancreatic tumor subtypes. Pan-Keratin antibodies mark epithelial cells in pancreatic adenocarcinomas while CD200 is a useful marker for neuroendocrine pancreatic tumors (4).
Glucose homeostasis is regulated by a variety of hormones including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (2). Antibodies to glucagon and proglucagon are useful markers for pancreatic α cells.
NKX6.1 is an important transcription factor in a network of transcription factors that are critical for pancreatic β cell development and maintenance (3). Antibodies to NKX6.1 are useful markers for β cells.
Pan-Keratin and CD200 antibodies are useful to mark and differentiate some pancreatic tumor subtypes. Pan-Keratin antibodies mark epithelial cells in pancreatic adenocarcinomas while CD200 is a useful marker for neuroendocrine pancreatic tumors (4).
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