Cellular Localization IF Antibody Sampler Kit #4753
Product Information
Kit Usage Information
Protocols
- 2128: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 2598: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 2639: Western Blotting, Immunofluorescence, Immunofluorescence
- 2679: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*
- 3868: Western Blotting, Immunofluorescence*, Flow
- 4499: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 4850: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 12349: Western Blotting, Immunofluorescence, ChIP Magnetic
- 46449: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence
Product Description
The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or two Western blots with each antibody.
Specificity / Sensitivity
Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.
Source / Purification
Rabbit monoclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to: the amino terminus of human β-tubulin, the sequence of human calnexin, residues surrounding Lys29 of human COX IV, the carboxy-terminal sequence of human histone H3 and human histone H2A, residues surrounding Pro671 of human NUP98, residues surrounding Thr298 of human fibrillarin, and residues near the amino terminus of LC3B. Mouse monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab5A protein.
Background
Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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