In spatial biology, multiplexing in immunohistochemistry (IHC) enables the simultaneous detection of multiple biomarkers within a single tissue section. This unravels insights into how cellular interactions influence the tissue microenvironment, deepening our understanding of disease progression and therapeutic responses.
SignalStar™ Multiplex IHC is a technology that uses antibodies, oligonucleotides, and fluorophores to interrogate cellular presence, location, function, and biomarker co-expression patterns. Multiple biomarkers can be amplified simultaneously in FFPE tissue with high sensitivity and specificity, providing results for up to eight biomarkers in two days.
SignalStar mIHC panels combine CST scientific expertise, our high-quality antibodies, and rigorous validation into an easy-to-use solution that enables you to generate multiplex spatial images and deepen your understanding of biology, even when you’re not a spatial expert. So you can easily design experiments and get answers to your most pressing scientific questions—fast.
You can design a SignalStar mIHC panel specific to your needs, or reference human-reactive and mouse-reactive multiplex IHC panel designs curated by CST scientists.
Click on the panels below to learn more.
Detect immune cell types in a human tumor microenvironment (TME).
| Biomarker | 相关性 |
|---|---|
| Pan-Keratin | Epithelium (identifies tumor cells) |
| CD3 | All T cells |
| CD4 | Helper T cells |
| CD8 | Cytotoxic T cells |
| FoxP3 | Regulatory T cells |
| CD68 | 巨噬细胞 |
| CD11c | Dendritic cells |
| CD20 | B 细胞 |
Gain a comprehensive understanding of T cell dynamics within the TME.
| Biomarker | 相关性 |
|---|---|
| Granzyme B | Cytotoxic potential of T cells |
| CD3 | All T cells |
| CD8 | Cytotoxic T cells |
| CD4 | Helper T cells |
| PD-1 | Stem-like T cells, exhausted T cells |
| TIM3 | Exhausted T cells |
| LAG3 | Exhausted T cells |
| TCF1 | Stem-like T cells |
Observe myeloid cells and relevant functional markers.
| Biomarker | 相关性 |
|---|---|
| CD11b | Myeloid cells |
| CD11c | M1-polarized macrophage |
| CD68 | 巨噬细胞 |
| CD206 | M2-polarized macrophage |
| CD163 | M2-polarized macrophage |
| HLA-DRA | M1-polarized macrophage |
| XCR1 | Conventional DC1 (CD8 T cell antigen) |
| CD86 | M1-polarized macrophage |
Gain detailed insights into immune cell infiltration versus immune exclusion within the TME.
| Biomarker | 相关性 |
|---|---|
| Pan-Keratin | Epithelium (identifies tumor cells) |
| CD3 | All T cells |
| CD8 | Cytotoxic T cells |
| FoxP3 | Regulatory T cells |
| F4/80 | 巨噬细胞 |
| CD11b | Myeloid cells |
| Ly6G | Neutrophils and MDSCs (myeloid-derived suppressor cells) |
| CD19 | B 细胞 |
Observe myeloid cells and relevant functional markers.
| Biomarker | 相关性 |
|---|---|
| CD11b | Myeloid cells |
| F4/80 | 巨噬细胞 |
| CD206 | M2-polarized macrophage |
| CD86 | M1-polarized macrophage |
| Arginase | M2-polarized macrophage |
| Ly6G | Neutrophils and polymorphonuclear MDSCs |
| SIRPɑ | Macrophage inhibitory receptor |
技术支持
Visit the Technical Support page to search for troubleshooting information and answers to technical questions.
Cell Signaling Technology、CST 和 SignalStar 均是 Cell Signaling Technology, Inc. 的商标。所有其他商标均属各自所有者专有。访问 cellsignal.com/trademarks 以了解更多信息。
第 10,781,477 号美国专利，外国对应物，以及由此而来的儿童专利。