Type I Interferon Induction and Signaling Antibody Sampler Kit #43573
Product Information
Kit Usage Information
Protocols
- 7074: Western Blotting
- 8826: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), ChIP Magnetic
- 11904: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 12390: Western Blotting
- 13014: Western Blotting
- 14994: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow, ChIP Magnetic, CUT&RUN Assay
- 37829: Western Blotting, Immunofluorescence*, Flow
- 37849: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin)
- 73671: Western Blotting
- 76684: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, ChIP Magnetic
Product Description
The Type I Interferon Induction and Signaling Antibody Sampler Kit provides an economical means of detecting the activation of pathways leading to upregulation of type I interferon (IFN), expression of IFN-β1, activation of signaling downstream of type I IFN, and expression of the MX1 interferon response gene, using phospho-specific and control antibodies. The kit includes enough antibodies to perform at least two western blot experiments.
Specificity / Sensitivity
Each antibody in the Type I Interferon Induction and Signaling Antibody Sampler Kit recognizes endogenous levels of its target protein. Phospho-IRF-3 (Ser386) (E7J8G) XP® Rabbit mAb recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser386. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb recognizes endogenous levels of IRF-7 protein only when phosphorylated at Ser477 and can also detect IRF-7 when dually phosphorylated at Ser477 and Ser479. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb may cross-react with unidentified proteins of 100 and 150 kDa. Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Ser727. Stat1 (D1K9Y) Rabbit mAb cross-reacts with an unidentified protein of 150 kDa. IRF-9 (D2T8M) Rabbit mAb cross-reacts with an unidentified protein of 95 kDa.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Pro115 of human IRF-7, Pro688 of human Stat1, Leu292 of human MX1, recombinant human IRF-3 protein, recombinant human IFN-β1 protein, and recombinant human IRF-9 protein. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Ser386 of human IRF-3, Ser477 of human IRF-7, and Ser727 of human Stat1.
Background
The innate immune system uses pattern recognition receptors (PRRs) that detect conserved pathogen-associated molecular patterns (PAMPs), such as cytoplasmic double-stranded RNA, to detect and initiate an immune response to viral infection. Detection of virus by PRRs leads to phosphorylation and nuclear translocation of IRF-3 and IRF-7, resulting in upregulation of type I interferons, which include IFN-α and IFN-β (1-3). Type I interferons signal through the interferon α/β receptor (IFNAR), leading to phosphorylation and activation of Stat1 and Stat2, which form a complex with IRF-9 (4,5). This complex translocates to the nucleus where it induces transcription of interferon response genes including viral restriction factors, such as MX1, that limit viral replication and propagation (4-7).
- Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.
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- Sato, M. et al. (2000) Immunity 13, 539-48.
- Fu, X.Y. et al. (1990) Proc Natl Acad Sci U S A 87, 8555-9.
- Qureshi, S.A. et al. (1995) Proc Natl Acad Sci U S A 92, 3829-33.
- Staeheli, P. et al. (1986) Cell 44, 147-58.
- Staeheli, P. and Haller, O. (1985) Mol Cell Biol 5, 2150-3.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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