SMAD2/3 Antibody Sampler Kit #12747
Product Information
Kit Usage Information
Protocols
- 3108: Western Blotting
- 5339: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow, ChIP Magnetic
- 7074: Western Blotting
- 8685: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq
- 9520: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic
- 9523: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq
- 38454: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq
Product Description
The SMAD2/3 Antibody Sampler Kit provides an economical means of detecting target proteins of the TGF-β signaling pathway. The kit includes enough antibody to perform two western blots with each primary antibody.
Specificity / Sensitivity
Each antibody in the SMAD2/3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. The total SMAD2, SMAD3, and SMAD4 antibodies detect their respective targets at endogenous levels.
Source / Purification
Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser465/467 of human SMAD2 or Ser423/425 of human SMAD3 protein. Total SMAD2, SMAD3, and SMAD4 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino termini of mouse SMAD2 and SMAD3, surrounding His198 of human SMAD2/3, or surrounding Asp165 of human SMAD4 protein.
Background
Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/8 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smad proteins by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smad proteins regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-4).
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