Phospho-VEGF Receptor 2 Antibody Sampler Kit #12599
Western blot analysis of extracts from CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992), using Phospho-VEGF Receptor 2 (Tyr996) Antibody (upper) or VEGF receptor 2 antibody (lower).
- Product Includes
- Related Products
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb #4991 | 20 µl | WB | H M | 230 | Rabbit IgG |
| Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474 | 20 µl | WB | H M | 230 | Rabbit |
| Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb #3817 | 20 µl | WB | H M | 230 | Rabbit IgG |
| Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb #3770 | 20 µl | WB, IP | H M | 230 | Rabbit IgG |
| VEGF Receptor 2 (D5B1) Rabbit mAb #9698 | 20 µl | WB, IP, IHC, IF, F | H M R | 210, 230 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | WB | Rab | Goat |
Product Information
Kit Usage Information
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Product Description
The Phospho-VEGF Receptor 2 Antibody Sampler Kit provides an economical means of evaluating the VEGFR2 tyrosine kinase and several phosphorylation sites that are involved in its activation. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
- Meyer, M. et al. (1999) EMBO J 18, 363-74.
- Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
- Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
- Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
- Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
- Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
- Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
- Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.
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