Phospho-MAPK Family Antibody Sampler Kit #9910
Product Information
Kit Usage Information
Protocols
- 4370: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 4511: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 4668: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
- 7074: Western Blotting
Product Description
The Phospho-MAPK Family Antibody Sampler Kit provides an economical means of evaluating the phosphorylation state of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibodies to perform two western blot experiments.
Specificity / Sensitivity
Phospho-p44/42 MAPK, Phospho-SAPK/JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated forms of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not significantly cross-react with other MAPK family members.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides surrounding Thr180/Tyr182 of human p38 MAPK, Thr202/Tyr204 of human p44 MAPK or Thr183/Tyr185 of human SAPK/JNK.
Background
p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).
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- Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
- Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
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限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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