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Render Timestamp: 2024-11-14T22:52:39.683Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-11-07 16:01:06.970
Product last modified at: 2024-11-11T13:15:40.405Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Mouse-Reactive STING Pathway Antibody Sampler Kit #16029

    Product Information

    Product Description

    The Mouse-Reactive STING Pathway Antibody Sampler Kit provides an economical means of detecting activation and expression of key components of the STING pathway using phospho-specific and control antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Mouse-Reactive STING Pathway Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-STING (Ser365) (D8W4F) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser365. Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated at Ser172. Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb may cross-react with phospho-IKKε. Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser396.

    Source / Purification

    Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Ala66 of mouse cGAS protein, Ser645 of human TBK1/NAK, residues near the carboxyl terminus of human IRF-3, and recombinant mouse STING protein. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding residues surrounding Ser365 of mouse STING protein, Ser172 of human TBK1, and Ser396 of human IRF-3.

    Background

    Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7,8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
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