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Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:13:12.185
Product last modified at: 2025-01-23T19:45:08.908Z
1% for the Planet 标识
PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Methyl-Histone H3 (Lys27) Antibody Sampler Kit #53312

Methyl-Histone H3 (Lys27) Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb.

To Purchase # 53312

  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #973320 µlWB, IHC, IF, F, ChIP, C&R, C&TH M R Mk17Rabbit IgG
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #972820 µlWB, IP, IF, F, ChIPH M R Mk17Rabbit IgG
Mono-Methyl-Histone H3 (Lys27) (D3R8N) Rabbit mAb #8493220 µlWB, IPH M R Mk17Rabbit IgG
Histone H3 (D1H2) XP® Rabbit mAb #449920 µlWB, IHC, IF, FH M R Mk17Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBRabGoat 

Product Information

Product Description

The Methyl-Histone H3 (Lys27) Antibody Sampler Kit provides an economical means of detecting levels of mono-, di-, and tri-methyl histone H3 Lys27 using methyl-specific and control histone H3 antibodies. The kit contains enough primary antibodies to perform at least two western blot experiments.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases, such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1, has shown that methylation is a reversible epigenetic marker (9).

Methylation of histone H3 Lys27 is generally associated with transcriptional repression and regulation of stem cell pluripotency and differentiation, through the regulation of facultative heterochromatin. Mono-, di-, and tri-methylation of histone H3 Lys27 are all mediated by polycomb repressor complex 2 (PRC2), which contains either the EZH1 or EZH2 methyltransferase proteins. Tri- and di-methyl-histone H3 Lys27 levels are highest at the promoters of polycomb-repressed genes. In addition, tri- and di-methyl-histone H3 Lys27 is found at the promoters of inactive, but transcriptionally poised genes that also contain the active tri-methyl-histone H3 Lys4 modification. Mono-methyl-histone H3 Lys27 is more widely dispersed and found in the bodies of active genes.

Pathways

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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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