IKK Isoform Antibody Sampler Kit #9966
Product Information
Kit Usage Information
Protocols
- 2690: Western Blotting
- 2695: Western Blotting
- 7074: Western Blotting
- 7076: Western Blotting
- 8943: Western Blotting, Immunoprecipitation (Magnetic)
- 11930: Western Blotting, Immunofluorescence
Product Description
The IKK Isoform Antibody Sampler Kit provides an economical means to investigate NFkappaB signaling within the cell. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Specificity / Sensitivity
IKKα, IKKβ, IKKγ, and IKKε antibodies detect endogenous levels of total IKKα, IKKβ, IKKγ and IKKε proteins, respectively. These antibodies do not cross-react with IKK subunits other than their specified target.
Source / Purification
The IKKβ (D30C6) Rabbit mAb was produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IKKβ protein. The IKKα (3G12) Mouse mAb was produced by immunizing animals with a recombinant protein specific to a fragment of human IKKα protein. The IKKγ (DA10-12) Mouse mAb was produced by immunizing animals with full length human GST-IKKγ protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human IKKγ. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
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限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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