Cell Cycle Regulation Antibody Sampler Kit II #9870
Western blot analysis of extracts from various cell lines using Cyclin B1 (D5C10) XP® Rabbit mAb.
- Product Includes
- Related Products
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539 | 20 µl | WB, IP, IF, F | H M R Mk | 34 | Rabbit |
| Cyclin A2 (BF683) Mouse mAb #4656 | 20 µl | WB | H | 55 | Mouse IgE |
| Cyclin B1 (D5C10) XP® Rabbit mAb #12231 | 20 µl | WB, IP, IF, F | H R | 55 | Rabbit IgG |
| Cyclin E2 Antibody #4132 | 20 µl | WB | H | 48 | Rabbit |
| Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 | 20 µl | WB, IF, F | H M R Mk Z | 17 | Rabbit IgG |
| Myt1 Antibody #4282 | 20 µl | WB, IHC | H M R | 60 to 70 | Rabbit |
| p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 | 20 µl | WB, IP, IHC, IF, F | H Mk | 21 | Rabbit IgG |
| Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910 | 20 µl | WB, IP | H | 95 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | WB | Rab | Goat | |
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | WB | M | Horse |
Product Information
Kit Usage Information
Protocols
- 2947: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 3377: Western Blotting, Immunofluorescence, Flow
- 4132: Western Blotting
- 4282: Western Blotting, Immunohistochemistry (Paraffin)
- 4539: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 4656: Western Blotting
- 4910: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 7076: Western Blotting
- 12231: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow
Product Description
The Cell Cycle Regulation Sampler Kit II provides an economical means of evaluating cell cycle proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.
Background
The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (1). Phosphorylation of cdc2 by the protein kinases Wee1 and Myt1 at Thr14 and Tyr15 results in inhibition of cdc2 (2,3). Progression through the G1/S checkpoint and initiation of DNA replication requires cyclin E; traversing the G2/M checkpoint to initiate mitosis requires cyclin B, and cyclin A may be required for both S-phase and M-phase (4). The versatile p21 cyclin-dependent kinase inhibitor, which interacts with several cyclin-CDK complexes to regulate cyclin-CDK during the cell cycle, is regulated by phosphorylation and ubiquitin-mediated degradation (5). Phosphorylation of histone H3 at Ser10 and neighboring residues correlates with chromosomal condensation, which is essential for segregation of chromosomes during mitosis (6).
- Norbury, C. et al. (1991) EMBO J 10, 3321-9.
- McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
- Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
- Pagano, M. et al. (1992) EMBO J 11, 961-71.
- Abbas, T. and Dutta, A. (2009) Nat Rev Cancer 9, 400-14.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
Pathways
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