Beclin-1 Complex Antibody Sampler Kit #59187
Western blot analysis of extracts from various cell lines using UVRAG (D2Q1Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). HCT 116 cells have been reported to contain a monoallelic frameshift mutation resulting in significantly reduced levels of endogenous UVRAG expression (11).
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| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Beclin-1 (D40C5) Rabbit mAb #3495 | 20 µl | WB, IP | H M R Mk | 60 | Rabbit IgG |
| PI3 Kinase Class III (D9A5) Rabbit mAb #4263 | 20 µl | WB, IP | H M R Mk | 100 | Rabbit |
| PIK3R4 Antibody #14580 | 20 µl | WB | H M R | 153 | Rabbit |
| Atg14 (D1A1N) Rabbit mAb #96752 | 20 µl | WB, IP | H M R | 65 | Rabbit IgG |
| UVRAG (D2Q1Z) Rabbit mAb #13115 | 20 µl | WB, IP | H M | 90 | Rabbit IgG |
| Rubicon (D9F7) Rabbit mAb #8465 | 20 µl | WB | H M | 130 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | WB | Rab | Goat |
Product Information
Kit Usage Information
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Product Description
The Beclin-1 Complex Antibody Sampler Kit provides an economical means of detecting proteins that are part of the Beclin-1 complexes. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Background
A number of studies have identified distinct complexes involving Beclin-1 and PI3K Kinase Class III with specific roles in autophagy and vesicle trafficking (1,2). These complexes commonly contain Beclin-1, PI3KC3/VSP34, and PIK3R4/VPS15 and function to catalyze the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate. Specificity of PI3KC3 activity is regulated by additional binding partners. Complex 1 contains Atg14 which is required for early stages of autophagosome nucleation (3,4). Complex 2 lacks Atg14, but instead contains UVRAG, and is important for autophagosome maturation and endocytic trafficking (4-6). A third complex, containing both UVRAG and Rubicon, negatively regulates canonical autophagy (7,8). Importantly, this complex containing Rubicon is critical for a related process of LC3-associated phagocytosis (LAP) in which extracellular pathogens binding to cell surface receptors are engulfed by a single membrane phagosome and degraded by the lysosome (9,10).
- Levine, B. and Kroemer, G. (2019) Cell 176, 11-42.
- Mei, Y. et al. (2016) Protein Sci 25, 1767-85.
- Sun, Q. et al. (2008) Proc Natl Acad Sci U S A 105, 19211-6.
- Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72.
- Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
- Liang, C. et al. (2008) Nat Cell Biol 10, 776-87.
- Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.
- Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
- Martinez, J. et al. (2015) Nat Cell Biol 17, 893-906.
- Heckmann, B.L. and Green, D.R. (2019) J Cell Sci 132(5). pii: jcs222984. doi: 10.1242/jcs.222984.
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