Western Blotting Application Solutions Kit #12957
From sample preparation to detection, everything you need for a more efficient and economical western blot is now found in one easy kit. We get our WB results using these reagents, now you can too.
- Product Includes
- Related Products
| Product Includes | Quantity |
|---|---|
| Cell Lysis Buffer (10X) #9803 | 15 ml |
| 30X Reducing Agent (1.25 M DTT) #14265 | 1 ml |
| 3X Blue Loading Buffer #56036 | 8 ml |
| Blue Prestained Protein Marker, Broad Range (11-250 kDa) #59329 | 100 µl |
| BSA #9998 | 12 g |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl |
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl |
| PMSF #8553 | 34.84 mg |
| Tris-Glycine SDS Running Buffer (10X) #4050 | 300 ml |
| Nitrocellulose Sandwiches #12369 | 10 Pack |
| Tris-Glycine Transfer Buffer (10X) #12539 | 300 ml |
| Tris Buffered Saline with Tween 20 (TBST-10X) #9997 | 300 ml |
| Nonfat Dry Milk #9999 | 50 g |
| SignalFire(TM) ECL Reagent A #46935 | 50 ml |
| SignalFire(TM) ECL Reagent B #74709 | 50 ml |
Product Information
Reagents Not Included
1. Primary Antibody
2. 1X PBS (#9808, 20X)
3. Reverse Osmosis Deionized (RODI) Water
4. Isopropyl alcohol
5. BCA Protein Assay (#7780) (optional)
6. Biotinylated Protein Ladder Detection Pack (#7727) (optional)
7. Methanol
8. 1X TBS (#12498, 10X) (optional)
9. Streptavidin-HRP (#3999) (if necessary)
Storage
All components in this kit are stable for at least 12 months past the reference date indicated on the component label when stored at the recommended temperature and left unused. Upon receipt, BSA and 3X Blue Loading Buffer should be removed from #13005S and stored at 4ºC and at room temperature, respectively. The 30X Reducing Agent and remaining #13005S components should be stored at -20ºC. All #13131S reagents should be stored at room temperature. IMPORTANT: Please refer to the primary antibody datasheet or product webpage for recommended primary antibody dilution buffer and recommended antibody dilution.
Protocol
Product Description
The Western Blotting Application Solutions Kit is designed to conveniently provide reagents needed for western blotting, from cell lysis to protein detection. The reagents in this kit are thoroughly validated with our primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents to run 10 mini-gels and complete western blot assays with either rabbit or mouse primary antibodies. All reagents in this kit are available individually.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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