Polycomb Group Antibody Sampler Kit #9788
Product Information
Kit Usage Information
Protocols
- 3737: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
- 5246: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
- 5694: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
- 6964: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
- 7074: Western Blotting
- 9733: Western Blotting, Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
- 13069: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
Product Description
The Polycomb Group Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group Proteins. The kit contains enough primary and secondary antibodies to perform two western mini-blot experiments.
Specificity / Sensitivity
Each antibody in the Polycomb Group Antibody Sampler Kit detects endogenous levels of target proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Arg354 of human Ezh2, Pro316 of human Ring1A protein, the human SUZ12 protein, the carboxy terminus of human Bmi1 protein, the amino terminus of histone H3 in which Lys27 is tri-methylated, or with recombinant protein specific to the full-length human RING1B protein, respectively.
Background
The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell-cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, Eed-Ezh2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinates histone H2A on Lys119 (6). PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of an additional catalytic subunit RING1B (also RING2 or RNF2) (7). PcG proteins play an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and are required for maintenance of adult hematopoietic and neural stem cells, as well as embryonic stem cells (3,4,8-10).
- Boyer, L.A. et al. (2006) Nature 441, 349-53.
- Lee, T.I. et al. (2006) Cell 125, 301-13.
- Park, I.K. et al. (2003) Nature 423, 302-5.
- Molofsky, A.V. et al. (2003) Nature 425, 962-7.
- Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
- Wang, H. et al. (2004) Nature 431, 873-8.
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
- Jacobs, J.J. et al. (1999) Nature 397, 164-8.
- Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.
限制使用
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