Neuronal Marker IF Antibody Sampler Kit #8572
Product Information
Kit Usage Information
Protocols
- 2837: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence
- 4760: Immunohistochemistry (Paraffin), Immunofluorescence
- 5568: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Immunofluorescence
- 5664: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence
- 12389: Western Blotting, Immunofluorescence
Product Description
The Neuronal Marker IF Antibody Sampler Kit provides an economical means for labeling neuronal structures by immunofluorescence (IF-F). This kit includes enough primary antibody to perform at least forty IF-F tests or two western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody in the Neuronal Marker IF Antibody Sampler Kit has been validated for IF-F, recognizes only its specific target, and does not cross-react with other family members. CNPase (D83E10) XP® Rabbit mAb recognizes endogenous levels of total CNPase protein. GFAP (D1F4Q) XP® Rabbit mAb detects endogenous levels of total GFAP protein. Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein. Neurofilament-L (C28E10) Rabbit mAb detects endogenous levels of total Neurofilament-L protein. β3-Tubulin (D71G9) XP® Rabbit mAb detects endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1. Expression of these proteins may vary in different cells and tissues.
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp395 of human GFAP protein, or the carboxy terminus of human β3-tubulin protein, or residues surrounding Val81 of human CNPase protein, or Glu450 of human Neurofilament-L protein.
Background
The antibodies in this kit serve as neuronal markers to determine protein localization in neurons. The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (2). Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells (3). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (4). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization and oligodendrocyte process outgrowth (5). GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used by investigators as a marker for intracranial and intraspinal tumors arising from astrocytes (6).
- Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.
- Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
- Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
- Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
- Lee, J. et al. (2005) J Cell Biol 170, 661-73.
- Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
限制使用
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