PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit #9120
Inquiry Info. # 9120
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Product Information
Kit Usage Information
Protocols
- 7003:
- 7074: Western Blotting
- 7075: Western Blotting
- 7727:
- 8727: Western Blotting, Immunofluorescence, Flow
- 9154: Western Blotting, Immunoprecipitation (Magnetic)
- 9160:
Product Description
The PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of MEK1/2 at Ser217/221.
Specificity / Sensitivity
Phospho-MEK1/2 (Ser217/221) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at serines 217 and 221. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221. MEK1/2 (D1A5) Rabbit mAb recognizes endogenous levels of total MEK1 and MEK2 proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 and synthetic peptide corresponding to residues surrounding Ala220 of human MEK1 protein.
Background
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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