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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Jak/Stat Pathway Inhibitors Antibody Sampler Kit

Jak/Stat Pathway Inhibitors Antibody Sampler Kit #8343

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Jak/Stat Pathway Inhibitors Antibody Sampler Kit provides an economical means to examine several inhibitors of Jak/Stat signaling, including PIAS1, PIAS3, PIAS4, SOCS1, SOCS2, and SOCS3. The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human SOCS2 protein, or recombinant protein specific to human SOCS3 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser550 of human PIAS1 protein, Pro590 of human PIAS3 protein, Lys59 of human PIAS4 protein, or near the carboxy terminus of human SOCS1 protein.

    Background

    Jak (Janus Kinase) and Stat (signal transducer and activator of transcription) proteins are utilized by receptors for a wide varity of ligands including cytokines, hormones, growth factors, and neurotransmitters (1). Jaks and Stats play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses, and stem cell differentiation (2-5). Therefore, regulation of Jak/Stat signaling is crucial to prevent aberrant signaling which can lead to disease progression. Two methods for regulating Jak/Stat signaling involve SOCS and PIAS proteins (6,7).The SOCS (suppressor or cytokine signaling) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway and consist of 8 known members, including the originally identified protein CIS1 (cytokine-inducible SH2-containing protein) and SOCS1-SOCS7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses (8-10). SOCS proteins are involved in regulating over 30 cytokines, including interleukins, growth hormone (GH), interferons, leptin, and leukemia inhibitory factor (7). SOCS1, also known as JAB (Janus Kinase binding protein) and SSI-1 (Stat-induced Stat inhibitor-1), shares the most homology with SOCS3 and both are highly induced by cytokines (7,11). Both SOCS1 and SOCS3 directly inhibit Jak activity; SOCS1 inhibits Jak through an interaction involving a phospohotyrosine located in the kinase activation loop; SOCS3 inhibits Jak via its SH2 domain (12,13). In addition to inhibiting Jak/Stat signaling, the SOCS box of SOCS1 and SOCS3 can trigger ubiquitin-mediated degradation of proteins within and outside the Jak/Stat pathway (14,15). SOCS2 is also incduced upon cytokine stimulation and the activity of SOCS2 has been predominately linked to GH and insulin-like growth fac
    1. Darnell, J.E. et al. (1994) Science 264, 1415-21.
    2. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
    3. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
    4. Cattaneo, E. et al. (1999) Trends Neurosci 22, 365-9.
    5. Su, L. et al. (1999) J Biol Chem 274, 31770-4.
    6. Shuai, K. (2006) Cell Res 16, 196-202.
    7. Croker, B.A. et al. (2008) Semin Cell Dev Biol 19, 414-22.
    8. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
    9. Chen, X.P. et al. (2000) Immunity 13, 287-90.
    10. Hilton, D.J. et al. (1998) Proc Natl Acad Sci U S A 95, 114-9.
    11. Starr, R. et al. (1997) Nature 387, 917-21.
    12. Yasukawa, H. et al. (1999) EMBO J 18, 1309-20.
    13. Sasaki, A. et al. (1999) Genes Cells 4, 339-51.
    14. Kamizono, S. et al. (2001) J Biol Chem 276, 12530-8.
    15. Rui, L. et al. (2002) J Biol Chem 277, 42394-8.
    16. Dey, B.R. et al. (1998) J Biol Chem 273, 24095-101.
    17. Liu, B. et al. (1998) Proc Natl Acad Sci U S A 95, 10626-31.
    18. Chung, C.D. et al. (1997) Science 278, 1803-5.
    19. Arora, T. et al. (2003) J Biol Chem 278, 21327-30.
    20. Nishida, T. et al. (2007) Biochem J 405, 481-8.
    21. Zhou, S. et al. (2008) J Biol Chem 283, 20137-48.

    Database Links

    UniProt ID: O15524 , Q8N2W9 , O14508 , Q9Y6X2 , O14543 , O75925


    Entrez-Gene Id: 8651 , 51588 , 8835 , 10401 , 9021 , 8554


    限制使用

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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