IRF Family Antibody Sampler Kit #57103
Product Information
Kit Usage Information
Protocols
- 4943: Western Blotting, Immunoprecipitation (Magnetic)
- 5628: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq
- 6948: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 8478: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, CUT&RUN Assay
- 11904: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 13014: Western Blotting
- 15106: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic
- 20261: Western Blotting, Immunoprecipitation (Magnetic)
- 76684: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, ChIP Magnetic
Product Description
The IRF Family Antibody Sampler Kit contains reagents to examine the total protein levels of various IRF isoforms. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each primary antibody.
Specificity / Sensitivity
Each antibody in the IRF Family Antibody Sampler Kit detects endogenous levels of its target protein. IRF-8 (D20D8) Rabbit mAb also detects an unknown background band at 80 kDa in some cell lines. IRF-9 (D2T8M) Rabbit mAb cross-reacts with an unidentified protein at 95 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro261 of human IRF-1, Pro115 of human IRF-7, and Gly65 of human IRF-8, or recombinant human IRF-3, mouse IRF-4, and human IRF-9 proteins. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Asp327 of human IRF-2, Gly121 of human IRF-6, and near the carboxy terminus of human IRF-5. Antibodies were purified by affinity chromatography.
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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