Hippo Signaling Antibody Sampler Kit #8579
Product Information
Kit Usage Information
Protocols
- 3477: Western Blotting, Immunoprecipitation (Agarose)
- 3682: Western Blotting, Immunoprecipitation (Magnetic)
- 3952: Western Blotting, Immunoprecipitation (Agarose)
- 7074: Western Blotting
- 8418: Western Blotting, Immunoprecipitation (Magnetic)
- 8699: Western Blotting, Immunohistochemistry (Paraffin)
- 13008: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin)
- 13301: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 13619: Western Blotting, Immunoprecipitation (Magnetic)
- 13730: Western Blotting, Immunoprecipitation (Magnetic)
Product Description
The Hippo Signaling Antibody Sampler Kit provides an economical means of detecting target proteins of the Hippo signaling pathway. The kit contains enough primary antibody to perform two western blots per primary.
Specificity / Sensitivity
Phospho-YAP (Ser397) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser397. This residue corresponds to Ser381 of YAP isoform 2, as reported by Zhao, B. et al. (2010) Genes Dev 24, 72-85 (9). Phospho-YAP (Ser127) (D9W2I) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser127. YAP/TAZ (D24E4) Rabbit mAb recognizes endogenous levels of total YAP and TAZ proteins. LATS1 (C66B5) Rabbit mAb recognizes endogenous levels of total LATS1 protein. Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb recognizes endogenous levels of MOB1 protein only when phosphorylated at Thr35. MOB1 (E1N9D) Rabbit mAb recognizes endogenous levels of total MOB1 protein. This antibody detects both MOB1A and MOB1B. Mst1 Antibody recognizes endogenous levels of total Mst1 protein. This antibody does not cross-react with Mst2-4. Mst2 Antibody recognizes endogenous levels of total Mst2 protein. This antibody does not cross-react with Mst1, Mst3, or Mst4. SAV1 (D6M6X) Rabbit mAb recognizes endogenous levels of total SAV1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Mst1 protein, or residues near the amino terminus of human Mst2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MOB1A protein, residues surrounding Gly180 of human LATS1 protein, residues near the carboxy terminus of human SAV1 protein, or residues surrounding Asp362 of human TAZ protein. Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr35 of human MOB1 protein, residues surrounding Ser397 of human YAP protein isoform 1, or residues surrounding Ser127 of human YAP protein.
Background
Hippo signaling is an evolutionarily conserved pathway that controls cell proliferation, apoptosis, and organ size in response to changing cell density levels (1,2). At relative low cell density, transcription co-activators YAP and TAZ bind transcription factors to induce expression of genes that favor cell growth and proliferation. As cell density increases, interaction between membrane-bound upstream hippo pathway regulators trigger activation of cytoplasmic kinases Mst1/2 and LATS1/2. Activated Mst kinase (the eponymous Hippo in Drosophila) associates with the adaptor Sav1 and phosphorylates MOB1 to activate LATS kinase, which phosphorylates YAP and TAZ to suppress cell proliferation (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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