GSK-3 Antibody Sampler Kit #9369
Product Information
Kit Usage Information
Protocols
- 4337: Western Blotting, Immunoprecipitation (Magnetic)
- 5558: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 7074: Western Blotting
- 8566: Western Blotting, Immunoprecipitation (Magnetic)
- 9316: Western Blotting, Immunohistochemistry (Paraffin)
- 12456: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
Product Description
The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Specificity / Sensitivity
Each antibody in the GSK3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Phospho-GSK-3ß (Ser9) (D85E12) XP® Rabbit mAb reacts with denatured components of bovine serum, including BSA.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser9 of human GSK-3ß, residues around Ser21 of human GSK-3α, or to the sequence of human GSK-3α protein or by immunizing animals with recombinant protein specific to the carboxy terminus of human GSK-3ß protein.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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