GSK-3 Antibody Sampler Kit #9369
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) using GSK-3β (D5C5Z) XP® Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-), and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
- Product Includes
- Related Products
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| GSK-3α (D80E6) Rabbit mAb #4337 | 20 µl | WB, IP | H M R Hm Mk | 51 | Rabbit IgG |
| Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 | 20 µl | WB, IHC | H M R Mk | 51 | Rabbit IgG |
| Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558 | 20 µl | WB, IP, IF, F | H M R Hm Mk | 46 | Rabbit IgG |
| Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb #8566 | 20 µl | WB, IP | H M R Hm Mk | 46 GSK-3beta, 51 GSK-3alpha | Rabbit IgG |
| GSK-3β (D5C5Z) XP® Rabbit mAb #12456 | 20 µl | WB, IP, IHC, IF, F | H M R Mk | 46 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | WB | Rab | Goat |
Product Information
Kit Usage Information
Protocols
- 4337: Western Blotting, Immunoprecipitation (Magnetic)
- 5558: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 7074: Western Blotting
- 8566: Western Blotting, Immunoprecipitation (Magnetic)
- 9316: Western Blotting, Immunohistochemistry (Paraffin)
- 12456: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
Product Description
The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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