4E-BP Antibody Sampler Kit #9955
Product Information
Kit Usage Information
Protocols
- 2845: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin)
- 2855: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 4923: Western Blotting, Immunofluorescence, Flow
- 7074: Western Blotting
- 9451: Western Blotting, Immunoprecipitation (Agarose)
- 9455: Western Blotting, Immunoprecipitation (Agarose)
- 9644: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
Product Description
The 4E-BP Antibody Sampler Kit provides an economical means to investigate regulation of cap-dependent translation within the cell. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Background
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
4E-BP2 and 4E-BP3 share high sequence homology with 4E-BP1, including conservation of the major FRAP/mTOR-dependent phosphorylation sites. Preliminary data suggests that phosphorylation of 4E-BP2 is regulated in a similar manner to that of 4E-BP1, although phosphorylation of this protein has not been as extensively studied (6).
4E-BP2 and 4E-BP3 share high sequence homology with 4E-BP1, including conservation of the major FRAP/mTOR-dependent phosphorylation sites. Preliminary data suggests that phosphorylation of 4E-BP2 is regulated in a similar manner to that of 4E-BP1, although phosphorylation of this protein has not been as extensively studied (6).
- Pause, A. et al. (1994) Nature 371, 762-7.
- Brunn, G.J. et al. (1997) Science 277, 99-101.
- Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
- Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
- Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.
- Lin, T.A. and Lawrence, J.C. (1996) J. Biol. Chem. 271, 30199-30204.
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