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Human CSF-1/M-CSF Recombinant Protein

Human CSF-1/M-CSF Recombinant Protein #8929

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Product Name

35913 Human CSF-1/M-CSF Recombinant Protein

Inquiry Info. # 8929

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Product Information

Product Usage Information

Reconstitution:

With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final hCSF-1/M-CSF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of hCSF-1/M-CSF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hCSF-1/M-CSF should be greater than 50 μg/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCSF-1/M-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa)	16-26
Purity	>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCSF-1/M-CSF. All lots are greater than 98% pure.
Endotoxin	Less than 0.01 ng endotoxin/1 μg hCSF-1/M-CSF.
Activity	The bioactivity of recombinant hCSF-1M/CSF was determined in a M-NFS-60 cell proliferation assay. The ED₅₀ of each lot is between 0.5-15.0 ng/ml.
Molecular Formula	Recombinant hCSF-1/M-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,403. DTT-reduced protein migrates as a 16-26 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 34-40 kDa protein. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino-terminal EEVSE of recombinant hCSF-1/M-CSF was verified by amino acid sequencing.

Source / Purification

Recombinant human CSF-1/M-CSF Glu33-Ser190 (Accession #P09603-3) was expressed in human 293 cells at Cell Signaling Technology.

Background

Colony stimulating factor 1 (CSF-1)/macrophage colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). CSF-1/M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). CSF-1/M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. CSF-1/M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). CSF-1/M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in CSF-1/M-CSF expression may contribute to cancer progression, and high plasma CSF-1/M-CSF levels are associated with rheumatoid arthritis (1,4,5).

Database Links

UniProt ID: P09603

Entrez-Gene Id: 1435

Product Citations: 1

Azzam, G. et al. (2013) PLoS One 8, e74297.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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