Human Interferon-α1 (hIFN-α1) #8927
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Product Information
Formulation
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIFN-α1. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Storage
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 18 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIFN-α1. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hIFN-α1. |
| Activity | The bioactivity of hIFN-α1 was determined in a virus protection assay. The ED50 of each lot is between 0.01-1.0 ng/ml. |
| Molecular Formula | Recombinant hIFN-α1 does not have a Met on the amino terminus and has a calculated MW of 19,386. DTT-reduced protein migrates as an 18 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal CDLPE of recombinant hIFN-α1 was verified by amino acid sequencing. |
Source / Purification
Recombinant human IFN-α1 (hIFN-α1) Cys24 - Asp189 (Accession # NP_076918) was produced in E. coli at Cell Signaling Technology.
Background
Interferon-α1 is a member of the Type I IFN (1) family best known for their antiviral activity. Most nucleated cells produce one or more Type I IFNs in response to viral infection (2). Secreted Type I IFN then induces viral protective responses in neighboring non-infected cells. Type I IFNs also enhance virus-induced apoptosis (3). Other IFN-α1 activities include enhancement of dendritic cell maturation and cytotoxic T cell activity (4). IFN-α1 binds to the IFN-αR1 and IFN-αR2 heterodimer (1). Intracellular signaling through the Jak/Stat pathway is best characterized (3). However, the PI3K, ERK, and p38 kinase pathways are also involved (5). The antiviral activities of the IFNs have led to their use in treating viral infections (4). Type I IFNs also appear to have an integral role in several autoimmune diseases (6).
- Nagai, T. et al. (2003) J Immunol 171, 5233-43.
- Stetson, D.B. and Medzhitov, R. (2006) Immunity 25, 373-81.
- Vilcek, J. (2006) Immunity 25, 343-8.
- Luft, T. et al. (2002) Int Immunol 14, 367-80.
- van Boxel-Dezaire, A.H. et al. (2006) Immunity 25, 361-72.
- Banchereau, J. and Pascual, V. (2006) Immunity 25, 383-92.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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