Human IFN-α1 Recombinant Protein #80435
Product Information
Storage
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 17-20 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU/ 1 μg hIFN-α1. |
| Activity | The bioactivity of recombinant IFN-α1 was determined in a TF-1 human erythroleukemic cell proliferation assay. The ED50 for this effect is 0.327 ng/mL. |
Source / Purification
Recombinant human IFN-α1 was expressed in 293 cells with a C-terminal His-tag and is supplied in a lyophilized form.
Background
Interferon-α1 is a member of the type I interferon (IFN) (1) family best known for their antiviral activity. Most nucleated cells produce one or more type I IFNs in response to viral infection (2). Secreted type I IFN then induces viral protective responses in neighboring non-infected cells. Type I IFNs also enhance virus-induced apoptosis (3). Other IFN-α1 activities include enhancement of dendritic cell maturation and cytotoxic T cell activity (4). IFN-α1 binds to the IFN-αR1 and IFN-αR2 heterodimer (1). Intracellular signaling through the Jak/Stat pathway is best characterized (3). However, the PI3K, ERK, and p38 kinase pathways are also involved (5). The antiviral activities of the IFNs have led to their use in treating viral infections (4). Type I IFNs also appear to have an integral role in several autoimmune diseases (6).
- Nagai, T. et al. (2003) J Immunol 171, 5233-43.
- Stetson, D.B. and Medzhitov, R. (2006) Immunity 25, 373-81.
- Vilcek, J. (2006) Immunity 25, 343-8.
- Luft, T. et al. (2002) Int Immunol 14, 367-80.
- van Boxel-Dezaire, A.H. et al. (2006) Immunity 25, 361-72.
- Banchereau, J. and Pascual, V. (2006) Immunity 25, 383-92.
限制使用
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