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  3. V5-Tag (D3H8Q) Rabbit mAb (BSA and Azide Free)
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V5-Tag (D3H8Q) Rabbit mAb (BSA and Azide Free) #58009

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Western Blotting Image 1: V5-Tag (D3H8Q) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with V5-tag fusion protein (+), using V5-Tag (D3H8Q) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457. Data were generated using the standard formulation of this product.
1/3

To Purchase # 58009

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #13202. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #13202

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

V5-Tag (D3H8Q) Rabbit mAb (BSA and Azide Free) recognizes transfected levels of recombinant protein containing the V5 epitope tag.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5 tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.
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