Render Target: SSR
Render Timestamp: 2025-03-29T12:11:19.648Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:06:52.787
Product last modified at: 2025-03-19T19:45:08.699Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

V5-Tag (D3H8Q) Rabbit mAb #13202

Filter:
  • WB
  • IP
  • IF
  • F
Western Blotting Image 1: V5-Tag (D3H8Q) Rabbit mAb
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with V5-tag fusion protein (+), using V5-Tag (D3H8Q) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.

To Purchase # 13202

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:500 - 1:2000
Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #58009.

Protocol

Specificity / Sensitivity

V5-Tag (D3H8Q) Rabbit mAb recognizes transfected levels of recombinant protein containing the V5 epitope tag.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5 tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
For Research Use Only. Not For Use In Diagnostic Procedures.
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