What is the expected yield of a CUT&Tag DNA library?

扩增的 CUT&Tag DNA 文库的预期产率取决于所用的 DNA 定量系统。

• If you are using a NanoDrop Spectrophotometer or QIAxpert System, the typical reading is between 10-20 ng/µL for histone targets and 5-12 ng/µL for non-histone targets. If the library concentration is lower than 3 ng/µL with the NanoDrop or QIAxpert System, please refer to the CUT&Tag Troubleshooting Guide before sequencing your samples.
• If you are using a Qubit Fluorometric Quantification System or PicoGreen dsDNA Assay, the typical reading is between 3-10 ng/µL for histone targets and could be lower than 1 ng/µL for non-histone targets.

However, low yield doesn't necessarily indicate a failure of the CUT&Tag experiment. We suggest continuing with next-generation sequencing (NGS) if the Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 positive control generates the expected library yield, or if the quantitative PCR (qPCR) QC of the DNA library generates a good signal-to-noise ratio. A low CUT&Tag DNA library yield shouldn't stop you from obtaining quality NGS data.

Related blog: CUT&Tag DNA Library Yield: What to Do if Yours Is Too Low to Detect with an Agilent Bioanalyzer or TapeStation System

Last updated: 2024 年 9 月 12 日