Technical support
Results (653)
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
SignalStar Multiplex IHC 试剂盒和试剂已针对抗体的荧光团配对和顺序作了优化。As tissues vary in quality and expression level of biomarkers, increasin...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ... The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers: Concanavalin A 磁珠可以很容易地凝集在一起，尤其是在细胞裂解时。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell... The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon... CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...我是否应该担心我的 Concanavalin A 磁珠在我的一些 CUT&RUN 实验中“聚集在一起”?
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
Can I modify the CST-recommended protocol for a given application?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. 这些策略包括：
- β-Actin (13E5) Rabbit mAb #4970
- β-Actin (D6A8) Rabbit mAb #...
Which total AMPKα antibody do you suggest for my western blot experiment?
The choice of AMPKα total antibody depends on the subunit of interest and the secondary antibody to be used. Cell Signaling Technology (CST) has five AMPKα antibodies that detect total protein:
- <...
Is my antibody recombinant?
We currently sell just over 5000 recombinant antibodies and we are actively working to have this information added to our product webpages. For now, the best place to find this information is on CiteA...
Can the Senescence β-Galactosidase Staining Kit #9860 be used with frozen tissue?
While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell ...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
CUT&RUN 是否偏向于常染色质或异染色质?
We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly ...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
尚未验证 SignalStar Multiplex IHC 试剂盒和试剂可与直接偶联物联合使用。The SignalStar™ Fluorescence Removal Kit The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l... SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust... The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe... 对于第 1 轮成像，在完成染色后 8 小时内成像时，染色应显示稳定信号。For Imaging Round 2, imaging should be performed as close to the completion of staini...Can I reuse PTMScan® antibody beads?
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. 我还能以某种方式在我的检测板中使用它吗？
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?