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How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Concanavalin A 磁珠可以很容易地凝集在一起，尤其是在细胞裂解时。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell... The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l... The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:我是否应该担心我的 Concanavalin A 磁珠在我的一些 CUT&RUN 实验中“聚集在一起”?
Can I reuse PTMScan® antibody beads?
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
您的 CUT&RUN 分析试剂盒是否适用于分离的胞核?
我们的 CUT&RUN 检测试剂盒经过优化，适用于整个细胞，并且适用于大量细胞系，包括贴壁和悬浮细胞。While it is not typically necessary to pre-isolate cell nucle...
Is my antibody recombinant?
We currently sell just over 5000 recombinant antibodies and we are actively working to have this information added to our product webpages. For now, the best place to find this information is on CiteA...
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...
What is the intended use of the PTMScan® Control peptides?
These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTMScan success and rep...
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. 有如下对照：
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Ra...
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samples. These are p44/4...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
Which S6 ribosomal protein antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several monoclonal antibodies for detecting ribosomal protein S6 that are validated for IF-IC with human samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Pro...
What is an appropriate positive control to include in this SignalStar™ assay? 是否需要多个对照？
Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. 我还能以某种方式在我的检测板中使用它吗？
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...
Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
We have several monoclonal antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IF-IC with mouse samples. Th...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
单个CUT&RUN反应中可以使用的最大细胞数量是多少?
我们发现每种测定法 100,000 细胞足以富集多种基因组修饰、转录因子和转录辅因子的靶基因座。However, one can increase the n...
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...
What types of Ras are recognized by your G12D and G12V mutant specific antibodies?
Our Ras (G12D Mutant Specific) (D8H7) Rabbit mAb #14429 and Ras (G12V Mutant Specific) (D2H12) Rabbit mAb #14412 will detect all three types of Ras (H-Ras, K-Ras, and N-Ras) if the respective mutation...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. 我如何知道这种过度染色是否正确？
在优化过程期间，我们已发现荧光染色可能比生色性染色显示更高的阳性%。To ensure any excess staining is specific, confirm that the correct sub...
Do I need to optimize the Tn5 tagmentation conditions for different cell types when performing CUT&Tag?
我们针对 CUT&Tag 测定法的优化标签化条件跨多种细胞系（贴壁细胞系和悬浮细胞系）良好发挥作用。We recommend using 2 μL of CUT&Tag pAG-Tn5 (Loaded) #79561 in...
Are any of your SARS-CoV-2 Spike Proteins suitable for cell culture?
Unfortunately, we have not tested our SARS-CoV-2 Spike Proteins in cell culture. Additionally, these products are not sterile, therefore we would not recommend using them in this manner.
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
尚未验证 SignalStar Multiplex IHC 试剂盒和试剂可与直接偶联物联合使用。The SignalStar™ Fluorescence Removal Kit The sequences of the synthetic peptides were based on modified peptides that we have consistently recovered and identified in human and mouse samples during our inte... The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1. Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ... We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
How were the sequences of the PTMScan® Control peptides chosen?
What is the extinction coefficient of my rabbit antibody?
What is the benefit of carrier vs. carrier free cytokines?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
What is your recommended procedure for antigen retrieval with a pressure cooker?
For antigen retrieval in a pressure cooker, bring slides submerged in buffer up to 125C, maintain for 30 seconds, then cool to 90C and maintain that for 10 sec. Remove the container from the device an...
Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spe...
What is the difference between phospho-Smad2 (Ser465/467) and phospho-Smad2 (Ser245/250/255)?
Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the c...