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Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex... SignalStar Multiplex IHC 试剂盒和试剂已针对抗体的荧光团配对和顺序作了优化。As tissues vary in quality and expression level of biomarkers, increasin... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
What is an appropriate positive control to include in this SignalStar™ assay? 是否需要多个对照？
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Does your Insulin (C27C9) Rabbit mAb #3014 detect proinsulin?
Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; https://www.uniprot.o...
Does the amino acid sequence of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (319-591) being longer than the amino acid sequence of other RBD products (318-541) affect its binding activity with ACE2?
The additional amino acids found in SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 should not affect the binding of RBD to ACE2 because the RBD region ...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser23...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
尚未验证 SignalStar Multiplex IHC 试剂盒和试剂可与直接偶联物联合使用。The SignalStar™ Fluorescence Removal Kit Seeing positive blue staining in your untreated cells is not a cause for concern. We have also observed positive blue staining in our negative controls in some cell types. For example, we always see t... The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1. As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By nature, the bead particle... 对于第 1 轮成像，在完成染色后 8 小时内成像时，染色应显示稳定信号。For Imaging Round 2, imaging should be performed as close to the completion of staini... 扩增的 CUT&Tag DNA 文库的预期产率取决于所用的 DNA 定量系统。
Is it normal to see blue staining in my untreated samples when using the Senescence β-Galactosidase Staining Kit #9860?
What is the extinction coefficient of my rabbit antibody?
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
What is the expected yield of a CUT&Tag DNA library?
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. 我还能以某种方式在我的检测板中使用它吗？
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...
Are the bands detected by your MT1-MMP (D1E4) Rabbit mAb #13130 the precursor (62 kDa) and active (50 kDa) forms?
Our MT1-MMP (D1E4) Rabbit mAb #13130 was produced with an antigenic peptide corresponding to residues surrounding Met293 of human MT1-MMP protein (see UniProt ID #P50281; https://www.uniprot.org/unipr...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. 我如何知道这种过度染色是否正确？
在优化过程期间，我们已发现荧光染色可能比生色性染色显示更高的阳性%。To ensure any excess staining is specific, confirm that the correct sub...
Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are:
- Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060
- Phosp...
Is PTMScan® compatible with SILAC?
PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ...
What is the intended use of the PTMScan® Control peptides?
These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTMScan success and rep...
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. 其中包括以下几类：
- Phospho-Akt (Ser473) (D9E) XP® Rabb...
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. 有如下对照：
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Ra...
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the rack with 10% bleach...