Technical support
Results (654)
What epitope is recognized by your LAMP1 (D4O1S) Mouse mAb #15665?
Our LAMP1 (D4O1S) Mouse mAb #15665 was produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein (see UniProt ID # P11279; https://www.uniprot.org/uniprot/P11279). The ...
Why does the volume of MitoTracker® vary between vials?
The volume of material varies between vials of the MitoTracker® products because of the small volume of the reagent added to the vial. When these vials dry, there can be localized vapor pressures that...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the SignalStar Multiplex IHC 试剂盒和试剂已针对抗体的荧光团配对和顺序作了优化。As tissues vary in quality and expression level of biomarkers, increasin... We have several antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2,... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is meant for use, in tan... The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l... SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust... Concanavalin A 磁珠可以很容易地凝集在一起，尤其是在细胞裂解时。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. 这些策略包括： We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IF-IC with mouse samples. These are:
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe... The higher band obtained with the TFE3 Antibody #14779 at approximately 80 kDa represents the sumoylated form of the TFE3 protein, which migrates higher than the unmodified form [see Miller, A.... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
We have found that a doublet may appear if samples are boiled prior to gel loading when performing a western blot with our Na,K-ATPase Antibody #3010. The protein structure of this target is extremel... For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop... We have several total Akt antibodies that are validated for IF-IC with mouse samples. These are:
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Can I purchase your Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product?
Can I reuse PTMScan® antibody beads?
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. 我还能以某种方式在我的检测板中使用它吗？
我是否应该担心我的 Concanavalin A 磁珠在我的一些 CUT&RUN 实验中“聚集在一起”?
What is your protocol for PNGase F treatment?
To deglycosylate a protein of interest in native conditions.
Reagents:
PNGase F, NEB P0704L (https://...Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
Why does the TFE3 Antibody #14779 detect multiple bands?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Why do I see a doublet when using your Na,K-ATPase Antibody #3010?
Can I order a custom formulation of an antibody?
Which total Akt antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?