Technical support

How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...

Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?

SignalStar Multiplex IHC 试剂盒和试剂已针对抗体的荧光团配对和顺序作了优化。As tissues vary in quality and expression level of biomarkers, increasin...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o...

Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?

The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...

Can I reuse PTMScan® antibody beads?

The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...

My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

我是否应该担心我的 Concanavalin A 磁珠在我的一些 CUT&RUN 实验中“聚集在一起”?

Concanavalin A 磁珠可以很容易地凝集在一起，尤其是在细胞裂解时。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...

Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

Can I order a custom formulation of an antibody?

For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...

Can the Senescence β-Galactosidase Staining Kit #9860 be used with frozen tissue?

While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell ...

How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?