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How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. 这些策略包括：My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
对于第 1 轮成像，在完成染色后 8 小时内成像时，染色应显示稳定信号。For Imaging Round 2, imaging should be performed as close to the completion of staini...
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
- Phospho-AMPKα (Thr172) (40H9) R...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
尚未验证 SignalStar Multiplex IHC 试剂盒和试剂可与直接偶联物联合使用。The SignalStar™ Fluorescence Removal Kit 在优化过程期间，我们已发现荧光染色可能比生色性染色显示更高的阳性%。To ensure any excess staining is specific, confirm that the correct sub... For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, we strongly recommend... The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejuni. The Cas9 (7A9... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... The sequences of the synthetic peptides were based on modified peptides that we have consistently recovered and identified in human and mouse samples during our inte... No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the rack with 10% bleach... If CST recommended buffers are being used for western blotting and high background or additional bands persist when probing for phospho-AMPK (Thr172), the issue could be related to the lysate sample. ... SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ... The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome samples (bacterial and ho... When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spe... We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. 其中包括以下几类：
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. 我如何知道这种过度染色是否正确？
Do you have a protocol for lysing in a 96-well plate (no sonication)?
Which Cas9 antibody is the most sensitive by western immunoblot?
What is an appropriate positive control to include in this SignalStar™ assay? 是否需要多个对照？
How were the sequences of the PTMScan® Control peptides chosen?
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
Why do I see high background by western blot when probing for phospho-AMPK (Thr172)?
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
Can I use mixed species samples for a PTMScan® experiment?
Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
What is the difference between phospho-Smad2 (Ser465/467) and phospho-Smad2 (Ser245/250/255)?
Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the c...
Is PTMScan® compatible with SILAC?
PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ...
Can I freeze the PTMScan® HS Magnetic Immunoaffinity Beads?
The PTMScan HS Magnetic Immunoaffinity Beads should not be frozen. We recommend storing this product at 4C. However, we have performed functional testing by IAP and subsequent mass spec analysis after...
What is the extinction coefficient of my rabbit antibody?
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
Why does MAFA (D2Z6N) Rabbit mAb #79737 recognize a band at approximately 48 kDa when the predicted molecular weight is approximately 37 kDa?
MAFA is a phosphoprotein. The multiple phosphorylation sites on this protein cause a mobility shift, resulting in the protein migrating slower by gel electrophoresis. This has been confirmed by treatm...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IF-IC with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
Does your Tau (D1M9X) XP® Rabbit mAb #46687 recognize all six Tau isoforms?
Our Tau (D1M9X) XP® Rabbit mAb #46687 is predicted to recognize 2N4R (tau40), 2N3R (tau39), 1N4R (tau46), 1N3R (tau37), 0N4R (tau24), and 0N3R (tau23), as all six isoforms include the antigenic peptid...
What are the two bands seen in western blot analysis using ANKRD15 (E9I4I) Rabbit mAb #69953?
When using ANKRD15 (E9I4I) Rabbit mAb #69953, two distinct bands are typically observed in western blotting experiments. These bands represent two different isoforms of the ANKRD15 protein:
- I...
Can your Streptavidin-HRP #3999 be used for ELISA?
Regrettably, we do not test our Streptavidin-HRP #3999 for use in ELISA . Therefore, we cannot provide any firm guarantees or a recommended dilution. However, it should work for ELISA with optimizatio...
Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. We recommend #14214 f...