Technical support
Results (653)
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
- Phospho-AMPKα (Thr172) (40H9) R...
您的 CUT&RUN 分析试剂盒是否适用于分离的胞核?
我们的 CUT&RUN 检测试剂盒经过优化，适用于整个细胞，并且适用于大量细胞系，包括贴壁和悬浮细胞。While it is not typically necessary to pre-isolate cell nucle...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved Caspas...
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...
是否可以通过 qPCR 分析 CUT&RUN 富集的 DNA，还是必须进行 NGseq 来分析我的数据？
Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis. The pr...
How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?
To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After digestion, you should ...
Has your Sox2 (D6D9) XP® Rabbit mAb #3579 been tested for cross-reactivity with other SOX family members?
We have not tested our Sox2 (D6D9) XP® Rabbit mAb #3579 for cross-reactivity with other SOX family transcription factors. However, sequence alignments have been done between the antigen used to produc...
Why can't spike-in DNA be used in CUT&Tag assays?
Tagmentation takes place within the nuclei. To minimize non-specific background, any excess Tn5 enzyme is washed away prior to activation. Similarly, spike-in DNA introduced into the reaction woul...
Is prewashing the Protein A Magnetic Beads #73778 and Protein G Magnetic Beads #70024 necessary for my IP experiment?
We recommend washing the Protein A and Protein G magnetic beads prior to use to help clear them of any potential increase in background due to the buffer in which they are provided.
Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?
Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammator...
What is KinomeView® Profiling?
KinomeView Profiling is a western blot based screening technique used in conjunction with motif antibodies to optimize conditions (time, dose, cell line, compound) for maximum differential response. T......
Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?
While the SignalStain® DAB Substrate Kit #8059 does have a recommended storage temperature of 4°C, we have found tha...
I am unable to use Triton X-100 as a permeabilizing agent in my immunofluorescence (IF) work. Are there any suitable alternatives that will yield comparable results?
Triton X-100 is a widely used nonionic surfactant for permeabilizing cell membranes prior to immunostaining, and it is a key component of our recommended immunofluorescence protocol. The European C...
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samples. These are p44/4...
Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. We recommend #14214 f...
Does the Human IL-22 Recombinant Protein #74808 contain any human or animal material and is it free of HIV/AIDS, HCV/HBC or other infectious material?
No materials of animal origin are used in the production of our Human IL-22 Recombinant Protein #74808. Therefore, additional testing for infectious material is not relevant.
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. 有如下对照：
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan)...
What is the concentration of the pAG-MNase Enzyme #57813?
The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.
什么是最适合我的 CUT&RUN NG-seq 实验的对照 DNA 样品？
在进行下游 NG-seq 分析的 CUT&RUN 检测时，可以使用富含 IgG 抗体的正常样品作为阴性对照。However, we have seen and heard from other scientists that norma...
Why was the Caspase-3 (8G10) Rabbit mAb #9665 discontinued?
Additional internal testing has shown that the Caspase-3 (8G10) Rabbit mAb #9665 recognizes both Caspase-3 and Caspase-7. When this antibody was released to the market, we were unaware of the homology...
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. 有如下对照：
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pa...
Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?
We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loading more protein (n...
Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several monoclonal antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IF-IC with human samples. Th...
What can I use as a loading control with the Cell Fractionation Kit #9038?
Due to the nature of the Cell Fractionation Kit #9038 and the localization of each of the proteins in the Loading Control Sampler Kit #5142, the proteins will not be equally expressed in all of the fr...
Do you have a methylene blue dot blot staining protocol?
We have found that the methylene blue staining of the amounts of DNA spotted when performing a DNA dot blot can be tricky and may need optimization.
1. It is very important to perform the methy...