Technical support
Results (652)
Will the Rab5A Antibody #2143 detect Ras-related proteins Rab-5B or Rab-5C?
The antigen for our Rab5A Antibody #2143 corresponds to residues surrounding Gly190 of human Ras-related protein Rab-5A (UniProt ID P20339). The antibody is predicted to cross-react with endogenous le...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).
Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?
While the SignalStain® DAB Substrate Kit #8059 does have a recommended storage temperature of 4°C, we have found tha...
How much sample is required for a PTMScan® experiment?
We recommend using 10 mg of protein per immunoaffinity purification (IAP) sample for our standard PTMScan kits. For the PTMScan HS product line, 1 mg of starting material is optimal. The minimum sampl...
Does your CD44 (156-3C11) Mouse mAb #3570 recognize all variants of human CD44?
The CD44 gene encodes 20 exons. Alternative splicing gives rise to CD44s and multiple CD44 variants (CD44v). CD44s is encoded by constant exons 1-5, 16-18, and 20. The CD44 variants are produced by...
Could you explain the techniques for normalization of DNA and why it is performed for CUT&RUN assays?
A CUT&RUN assay allows signal normalization among samples or among experiments by adding spike-in DNA in the stop buffer. Since you add the same amount of spike-in DNA in the assay, the signal fro...
Do you have any tips for using FACS-isolated cells in CUT&RUN experiments?
We have no specific tips for using FACS-isolated cells in CUT&RUN experiments. Like a cultured cell suspension, if your isolated cells are in less than 40ul PBS, you can move directly to Concanava...
Can I separate nuclear and cytoskeletal proteins using your Cell Fractionation Kit #9038?
Unfortunately, separation of nuclear and cytoskeletal proteins is not possible using our Cell Fractionation Kit #9038. Because cytoskeletal proteins are involved in the stabilization and localization ...
Is the Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) #9872 RNase free?
Our Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) #9872 (both L and S sizes) is tested for sterility and endotoxin levels. All new lots are subjected to a 2 week sterility test and also tested to...
The DNA yield for my CUT&RUN experiment seems a bit low compared to what I was getting in my ChIP experiments. How can I improve the yield?
A ChIP experiment uses 4M cells per reaction while a CUT&RUN assay only uses 5K to 100K starting cells, so it is expected to see a lower DNA yield from a CUT&RUN assay than from ChIP experimen...
Can I use DNA affinity columns to purify CUT&RUN DNA for transcription factors?
Yes, we recommend using our ...
Will particulates in the protein substrate tubes of the GTPase kits negatively affect the results of the assay?
It is not uncommon to see particulates in the protein substrate tubes provided with the GTPase kits. After extensive testing, we have found that these are not a cause for concern. We recommend briefly...
What is the pH of your SignalStain® EDTA Unmasking Solution (10X) #14747?
The pH of our SignalStain EDTA Unmasking Solution (10X) #14747 is 8.0.
Are your siRNAs pools or single duplexes?
All of our siRNAs are single duplexes. We discontinued pools a number of years ago to avoid off-target effects.
What is the depth of sequencing reads per sample required in a CUT&RUN assay?
We suggest a sequencing depth of 3-5 million reads per sample. Because of the very low background signal generated in CUT&RUN, this sequencing depth is usually sufficient for histone modifications...
What is the percentage of SDS in the ChIP Sonication Nuclear Lysis Buffer #28778?
The ChIP Sonication Nuclear Lysis Buffer #28778 included in the SimpleChIP® Sonication Cell and Nuclear Lysis Buffers #81804 contains 0.1% SDS.
What is the species cross-reactivity of your Anti-rabbit IgG, HRP-linked Antibody #7074?
It has been determined that our Anti-rabbit IgG, HRP-linked Antibody #7074 has the following low cross-reactivities with species other than rabbit:
- Guinea Pig IgG, Mouse IgG - 3%
- Cat I...
What is the composition of your Lysis/Binding/Wash Buffer #11524?
The composition of our Lysis/Binding/Wash Buffer #11524 is as follows: 25 mM Tris-HCl, pH 7.2, 150 mM NaCl, 5 mM MgCl2, 1% NP-40 and 5% glycerol.
Where can I find example data for your PTMScan® kits?
We provide example data for our PTMScan enrichment kits on this webpage:
https://www.cellsignal.com/applications/proteomics/ptmscan-motif-antibody-kits
To the right of each product listi...
Can you enrich for more than one PTM in a PTMScan® sample?
Yes, the flow through from a PTMScan experiment can be saved and stored at -80C for future enrichments. We have performed 5 different PTM enrichments sequentially from one sample. The limitation would...
How are your antibody concentrations determined?
We determine the concentration of our antibodies by measuring the absorbance at 280nm (OD280).
Are the Protein G Agarose Beads #37478 compatible with goat antibodies?
While we have not tested our Protein G Agarose Beads #37478 with goat antibodies in-house, goat IgG has a strong binding affinity to protein G. Therefore, our Protein G Agarose Beads should be compati...
Why does the band size change with tunicamycin treatment when using your ATF-6 (D4Z8V) Rabbit mAb #65880 for western blot?
The product webpage for our ATF-6 (D4Z8V) Rabbit mAb #65880 shows a molecular weight shift from 100 kDa to 90 kDa following treatment with tunicamycin, an agent which induces ER stress. The short, 1 h...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
Why am I unable to see phalloidin staining in my methanol-fixed and permeabilized samples?
Phalloidin is only able to bind the native quaternary structure of F-actin. In order to retain the quaternary protein structure, formaldehyde fixation should be utilized. Methanol and other alcohols d...
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
SignalStar Multiplex IHC 试剂盒和试剂已针对抗体的荧光团配对和顺序作了优化。As tissues vary in quality and expression level of biomarkers, increasin...
Do you observe any differences in CUT&RUN assay performance among different cell lines?
我们已对数十种培养的贴壁和悬浮细胞系（包括人和小鼠）进行了 CUT&RUN 分析。So far we have not noticed any significant difference in performance between these cell li...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
What active Rho isoform will your Active Rho Detection Kit #8820 detect?
The antibody in our Active Rho Detection Kit #8820 detects RhoA, RhoB, and RhoC. Regrettably, there is no way to distinguish between the three proteins.
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...