Technical support
Results (653)
How do I contact the CST Customer Service team?
For our US based customers, our customer service team is available Monday through Friday from 上午 8:30 to 8 PM ET. You can reach the team by email at [email protected] or by phone at 877-616-2355.
Can your Protease/Phosphatase Inhibitor Cocktail (100X) #5872 be used for mass spectrometry?
Yes, our Protease/Phosphatase Inhibitor Cocktail (100X) #5872 can be used in mass spectrometry (MS) experiments. This cocktail does not contain AEBSF, which can cause peaks to shift.
Why does the TFE3 Antibody #14779 detect multiple bands?
The higher band obtained with the TFE3 Antibody #14779 at approximately 80 kDa represents the sumoylated form of the TFE3 protein, which migrates higher than the unmodified form [see Miller, A....
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. 有如下对照：
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan)...
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
- Phospho-Akt (Ser473) (D9E) XP® Rabb...
您建议使用哪种 DNA 库制备试剂盒来进行 CUT&RUN 分析?
We recommend using our SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #567...
您如何确定 3 到 5 百万个测序读数的 NG-seq 测序深度足以进行 CUT&RUN 分析？
这一决定基于我们对数百份 CUT&RUN 样品的分析。We found that a sequencing depth lower than 3 million generated too few peaks and that the number of peaks increased with de...
Why is the PTMScan® LysC Protease #84748 digestion performed at room temperature and not at 37°C?
The digestion is performed at room temperature to minimize carbamylation (also known as carbamoylation) of the sample peptides in the urea buffer, which can interfere with downstream PTMScan enrichmen...
采用 CST CUT&Tag 试剂盒的反应中我可以使用的最少细胞数目是多少？
We have shown that our CUT&Tag Assay Kit #77552 works with as few as 5,000-10,000 cells for histone modifications and 20,000 cells for transcription factors and cofactors.
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
What is an appropriate positive control to include in this SignalStar™ assay? 是否需要多个对照？
Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som...
Can the Senescence β-Galactosidase Staining Kit #9860 be used with frozen tissue?
While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell ...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
CST 如何确保其抗体的品质？
CST scientists test all our antibodies in relevant applications such as western blotting, immunoprecipitation, immunofluorescence (ICC), immunohistochemistry, flow cytometry, ELISA, and chromatin immu...
如果说明书上某些物种未列示为与 CST 抗体交叉反应，则这些物种是否经过测试？
可能经过检测。CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does not show cross-reac...
How should I store my antibody?
Proper storage of your antibody may vary depending on the product's storage buffer and antibody conjugation. Therefore, please refer to the "Storage" section of the product webpage or to...
What is the fewest number of cells I can use in a reaction with your CUT&RUN Assay Kit #86652?
We have shown that our CUT&RUN Assay Kit #86652 works with as few as 5,000-10,000 cells for histo...
Why do I see a doublet when using your Na,K-ATPase Antibody #3010?
We have found that a doublet may appear if samples are boiled prior to gel loading when performing a western blot with our Na,K-ATPase Antibody #3010. The protein structure of this target is extremel...
是否可以对心脏、大脑或发育中的小鼠组织等组织样本进行 CUT&RUN 测定？
可以。We have shown that our CUT&RUN Assay Kit #86652 works with mouse liver, mouse brain, and mouse heart tissues with as little as 1mg of tissue for each CUT&RUN reaction. Fresh tissue sample...
Can I use CST antibodies for fluorescent detection in FFPE tissue?
At CST we do not typically validate antibodies using indirect fluorescent detection in formalin fixed, paraffin embedded (FFPE) tissue. If a product is recommended for use in chromogenic IHC, denoted ...
CUT&RUN DNA 的预期大小分布是多少?
When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosomal ladder of DNA frag...
Which total Akt antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several total Akt antibodies that are validated for IF-IC with mouse samples. These are:
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Rabbit mAb #4685
- Akt Antibo...
什么是最适合我的 CUT&RUN NG-seq 实验的对照 DNA 样品？
在进行下游 NG-seq 分析的 CUT&RUN 检测时，可以使用富含 IgG 抗体的正常样品作为阴性对照。However, we have seen and heard from other scientists that norma...
NG-seq 分析需要多少 CUT&RUN DNA?
The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. 每个反应的 CUT&RUN DNA 的典型产量为 0.6 至 6 ng。Our We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fractionating, we recommen... Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis. The pr... We have used LysC in conjunction with trypsin for TMT-labeling-based studies to reduce miscleavages. For TMT-based workflows, we recommend a higher LysC:substrate ratio of 1:100 compared to the 1:250 ... For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. 我们建议使用胰蛋白酶分离细胞。Alternatively, Accutase Cell Dissociation Reagent... We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. 有如下对照：
Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?
是否可以通过 qPCR 分析 CUT&RUN 富集的 DNA，还是必须进行 NGseq 来分析我的数据？
Can I use PTMScan® LysC Protease #84748 for tandem mass tag (TMT) labeling?
What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
What are the methods for PTMScan® mass spectrometry?
The methods for the LC and MS are shown below:
| <... |