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How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l... 在优化过程期间，我们已发现荧光染色可能比生色性染色显示更高的阳性%。To ensure any excess staining is specific, confirm that the correct sub... Concanavalin A 磁珠可以很容易地凝集在一起，尤其是在细胞裂解时。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell... 对于第 1 轮成像，在完成染色后 8 小时内成像时，染色应显示稳定信号。For Imaging Round 2, imaging should be performed as close to the completion of staini... 我们的 CUT&RUN 检测试剂盒经过优化，适用于整个细胞，并且适用于大量细胞系，包括贴壁和悬浮细胞。While it is not typically necessary to pre-isolate cell nucle... PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ... 野生型 α-裂解型蛋白酶 (WaLP) 是一种丝氨酸肽链内切酶，这种酶会裂解丙氨酸、丝氨酸、苏氨酸和缬氨酸等氨基酸的羧基末端侧。 When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am... We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex... SignalStar Multiplex IHC 试剂盒和试剂已针对抗体的荧光团配对和顺序作了优化。As tissues vary in quality and expression level of biomarkers, increasin... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. 这些策略包括：Can I reuse PTMScan® antibody beads?
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. 我如何知道这种过度染色是否正确？
我是否应该担心我的 Concanavalin A 磁珠在我的一些 CUT&RUN 实验中“聚集在一起”?
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
您的 CUT&RUN 分析试剂盒是否适用于分离的胞核?
Is PTMScan® compatible with SILAC?
What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?
WaLP recognizes and cleaves SUM...What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
What is an appropriate positive control to include in this SignalStar™ assay? 是否需要多个对照？
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
尚未验证 SignalStar Multiplex IHC 试剂盒和试剂可与直接偶联物联合使用。The SignalStar™ Fluorescence Removal Kit CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o... SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
Can I modify the CST-recommended protocol for a given application?
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. 我还能以某种方式在我的检测板中使用它吗？
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. 其中包括以下几类：
- Phospho-Akt (Ser473) (D9E) XP® Rabb...
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...
What is the extinction coefficient of my rabbit antibody?
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ...
In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?
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International Units (IU) and specific activity are two different ways of measuring... |