PTMScan® Direct Apoptosis/Autophagy Service 允许对细胞凋亡或细胞自噬通路内或者与之相关的一组确定的蛋白质位点进行靶向筛选和定量分析,而且该组蛋白质位点对药物发现、生物标记物发现或诊断学发展至关重要。下表列出了可使用 PTMScan® Direct Apoptosis/Autophagy Service 进行监测的蛋白质位点列表。
总而言之,PTMScan® Direct Apoptosis/Autophagy 服务提供了一种可定性识别和定量分析 175 个特有的磷酸化位点和 Caspase 剪切的 100 种蛋白质的稳健方法。
细胞凋亡/细胞自噬蛋白质位点 | |||
---|---|---|---|
细胞凋亡/细胞自噬蛋白质位点 | |||
α-Tubulin | Akt1 | Akt1 (Ser473) | Akt1 (Ser477) |
Akt1 (Thr305) | Akt1 (Thr308) | Akt1 (Thr312) | Akt1 (Thr479) |
Akt1 (Tyr315) | Akt1 (Tyr474) | Akt2 (Ser302) | Akt2 (Thr306) |
Akt2 (Thr309) | Akt2 (Thr313) | Akt2 (Tyr316) | Akt3 (Ser472) |
Akt3 (Ser474) | Akt3 (Ser476) | Akt3 (Thr302) | Akt3 (Thr305) |
Akt3 (Thr309) | Akt3 (Tyr473) | ANXA5 | API5 |
ARC | ATG12 | ATG12 (Ser41) | ATG12 (Thr30) |
ATG3 | ATG4C | ATG7 | ATG9A |
β-Actin | BAD (Ser97) | BAD (Ser99) | Bak1 |
Bcl-11B | Bcl-9L | Bcl-9L (Thr1370) | Bcl-xL |
Bid | BIRC6 | Casp2 | Casp3 |
Casp3 (Asp175) | Casp3 (Ser176) | Casp6 | Casp6 (Asp179) |
Casp7 | Casp7 (Asp198) | Casp7 (Ser199) | Casp8 (Asp374) |
Casp9 | Casp9 (Asp315) | Casp9 (Ser302) | Casp9 (Ser307) |
CCAR1 | CCDC72 | CIAPIN1 | CLU |
CYC1 | CYCS | DBC-1 | DBC-1 (Ser124) |
DDAH2 | DNA-PK | ERK1 (Thr198) | ERK1 (Thr202) |
ERK1 (Tyr204) | ERK2 (Thr181) | ERK2 (Thr185) | ERK2 (Thr190) |
ERK2 (Tyr187) | Fas | Fas (Asp269) | FYCO1 |
G6PI | GAPDH | GRP94 | GSK3A (Thr19) |
H3 | HELLS | IκBα (Ser32) | IκBα (Ser36) |
IκBα (Tyr42) | IκBε (Ser18) | IκBε (Ser22) | IκBε (Ser25) |
IKKβ | Jun (Ser58) | Jun (Ser63) | Jun (Ser73) |
Jun (Thr62) | JunD (Ser100) | JunD (Ser90) | LDH-A |
MIF | MMRP19 | mTOR | mTOR (Ser2478) |
mTOR (Ser2481) | mTOR (Thr2471) | mTOR (Thr2473) | mTOR (Thr2474) |
Myc | NF-κB p105 | NF-κB p105 (Ser923) | NF-κB p105 (Ser927) |
NF-κB p105 (Ser932) | NF-κB p65 | NF-κB p65 (Asp512) | NF-κB p65 (Ser536) |
Nik (Thr764) | Nudt2 | p400 | PAR-4 (Asp131) |
PARP1 | PDCD10 | PDCD5 | PDCD8 |
PDCL3 | PHLDA1 | PI4K2A (Thr76) | PIK3C2A |
PIK3C3 | PIK3CA | PIK3CA (Ser507) | PIK3CA (Tyr508) |
PIK3CB | PIK3CD | PIK4CA | PIP5K2A |
PKCA | PKCA (Thr494) | PKCA (Thr501) | PKCB |
PKCB (Thr497) | PKCB (Thr500) | PKCB (Thr504) | PKCB (Tyr507) |
PKCH | PKCT (Thr275) | PKD1 (Ser473) | PKD1 (Ser738) |
PKD2 (Ser706) | PKD3 (Ser731) | PRAS40 | PRAS40 (Ser203) |
PRAS40 (Thr266) | RABGAP1 | Raptor | Raptor (Ser877) |
Raptor (Ser886) | Raptor (Thr883) | Raptor (Thr889) | Rictor |
ROCK1 | ROCK2 | RUSC1 | SH3GLB1 |
SH3GLB2 | SHP-2 | SHP-2 (Ser580) | SHP-2 (Tyr584) |
SOD1 | STAG1 | Survivin | Survivin (Asp72) |
TNF-R1 | ULK1 (Ser556) | ULK4 (Thr645) | XIAP |
ZNF768 (Ser17) | ZNF768 (Ser18) | ZNF768 (Ser23) |