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Protein G Agarose Beads #37478

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Immunoprecipitation Image 1: Protein G Agarose Beads
Immunoprecipitation (IP) of COX IV from HeLa cells using COX IV (4D11-B3-E8) Mouse mAb #11967 and Protein G Agarose Beads. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using COX IV (3E11) Rabbit mAb #4850.

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Product Information

Product Usage Information

Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.

Storage

Supplied as a 50% slurry in 20% ethanol solution. Store at 4°C. This product is stable for 12 months.

Protocol

Product Description

Protein G Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein G is covalently coupled to agarose beads. Protein G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

Product Specifications:
Bead Diameter: ~50-150 micron per bead
Binding Capacity: ~20 mg human IgG/ml

Specificity / Sensitivity


Species Reactivity:

All Species Expected
For Research Use Only. Not For Use In Diagnostic Procedures.
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