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Myc-Tag (9B11) Mouse mAb (Sepharose® Bead Conjugate) #3400
Filter:
- IP
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a kappa |
Application Key:
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Myc-Tag (9B111) Mouse mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of Myc-tagged recombinant proteins.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:20 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Protocol
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb (Sepharose® Bead Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (9B11) Mouse mAb (Sepharose® Bead Conjugate) detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.
The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin ~90kDa.
The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin ~90kDa.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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