Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) #5698

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Mouse IgG2a kappa

Application Key:

IP-Immunoprecipitation

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of Myc epitope-tagged proteins.

Product Usage Information

Application	Dilution
Immunoprecipitation	1:20

Storage

Supplied in PBS Buffer (pH 7.2), 0.1% Tween^® 20. Store at 4°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.

The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin ~90kDa.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems (1).

Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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