渲染靶标:SSR
Render Timestamp: 2025-03-15T01:01:57.845Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2025-01-28 06:01:09.015
Product last modified at: 2025-01-28T08:00:59.893Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) #5939

Filter:
  • IP
Immunoprecipitation Image 1: AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate)
Immunoprecipitation of Jurkat, NIH/3T3, and HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 and AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using AIF (D39D2) XP® Rabbit mAb #5318 and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127.

To Purchase # 5939

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 67
Source/Isotype Rabbit IgG
Application Key:
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose beads. AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of AIF. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated AIF (D39D2) XP® Rabbit mAb #5318.
MW (kDa) 67

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) recognizes endogenous levels of total AIF protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Bovine, Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to redidues surrounding Ala520 of human AIF protein.

Background

Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
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