渲染靶标:SSR
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4/3/2025, 2:15:42 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:11:04.204
Product last modified at: 2025-01-01T09:02:20.835Z
1% for the Planet 标识
PDP - Template Name: siRNA
PDP - Template ID: *******aa36529

SignalSilence® Ezh2 siRNA I #6509

SignalSilence® Ezh2 siRNA I: Image 1
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Ezh2 siRNA I (+), using Ezh2 (D2C9) XP® Rabbit mAb #5246 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Ezh2 (D2C9) XP® Rabbit mAb confirms silencing of Bak expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

To Purchase # 6509

Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.

Supporting Data

REACTIVITY H
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

CST recommends transfection with 100 nM Ezh2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Storage

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® Ezh2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Ezh2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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