Render Target: SSR
Render Timestamp:
4/4/2025, 2:33:23 AM EDT
4/4/2025, 6:33:23 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:19:51.866
Product last modified at: 2025-03-06T09:00:18.716Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

TIM-3 (D3M9R) & CO-0094-647 SignalStar Oligo-Antibody Pair #25260

Filter:
  • IHC
Immunohistochemistry Image 1: TIM-3 (D3M9R) & CO-0094-647 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse lung using CD20 (E3N7O) & CO-0134-488 SignalStar™ Oligo-Antibody Pair #18644 (yellow), TIM-3 (D3M9R) & CO-0094-594 SignalStar™ Oligo-Antibody Pair #55557 (cyan), CD68 (E3O7V) & CO-0096-647 SignalStar™ Oligo-Antibody Pair #58388 (red), F4/80 (D2S9R) & CO-0042-750 SignalStar™ Oligo-Antibody Pair #51924 (magenta), and Pan-Keratin (Type I) (E6S1S) & CO-0072-594 SignalStar™ Oligo-Antibody Pair #30754 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

Order Information # 25260

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
TIM-3 (D3M9R) XP® Rabbit mAb (SignalStar™ Conjugate 0094) #5172350 µlMRabbit IgG
Complementary Oligo (CO-0094-647) #8393422 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

TIM-3 (D3M9R) XP® Rabbit mAb (SignalStar™ Conjugate 0094) recognizes endogenous levels of total TIM-3 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro137 of mouse TIM-3 protein.

Background

T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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