渲染靶标：SSR
Render Timestamp: 2025-03-18T07:17:26.681Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2025-03-05 20:27:11.359
Product last modified at: 2025-03-06T09:00:16.700Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7
  1. 产品 /
  2. SignalStar™ Multiplex IHC 试剂盒与试剂 /
  3. SIRPα/SHPS1 (D6I3M) & CO-0034-750 SignalStar™ Oligo-Antibody Pair

SIRPα/SHPS1 (D6I3M) & CO-0034-750 SignalStar™ Oligo-Antibody Pair #35593

筛选器：
  • IHC
Immunohistochemistry Image 1: SIRPα/SHPS1 (D6I3M) & CO-0034-750 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using SIRPα/SHPS1 (D6I3M) & CO-0034-594 SignalStar™ Oligo-Antibody Pair #60004 (yellow), Vimentin (D21H3) & CO-0012-488 SignalStar™ Oligo-Antibody Pair #96317 (green), CD45 (Intracellular Domain) (D9M8I) & CO-0013-647 SignalStar™ Oligo-Antibody Pair #32740 (red), and CD44 (E7K2Y) & CO-0030-750 SignalStar™ Oligo-Antibody Pair #18266 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
1/10

Order Information # 35593

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • 产品包括
  • 相关产品
产品包括体积反应性同型
SIRPα/SHPS1 (D6I3M) Rabbit mAb (SignalStar™ Conjugate 0034) #6954550 µlH MRabbit IgG
Complementary Oligo (CO-0034-750) #8004822 µl

产品信息

产品使用信息

应用 稀释度
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

保存

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. 切勿分装抗体。‭ All components in this kit are stable for at least 12 months when stored at the recommended temperature.

产品说明

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

实验步骤

特异性/灵敏度

SIRPα/SHPS1 (D6I3M) Rabbit mAb (SignalStar™ Conjugate 0034) recognizes endogenous levels of total SHPS1 protein. This antibody recognizes both large and small isoforms of murine mSHPS1/SIRPα.

物种反应性：

Human, Mouse

来源/纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro413 of human SIRPα/SHPS1 protein.

背景

SHP-substrate 1 (SHPS1, SIRPα) is a single-pass membrane protein and member of both the immunoglobulin superfamily and the signal regulatory protein (SIRP) family. Following growth hormone stimulation or integrin binding, SHPS1 is phosphorylated at several tyrosine residues within its cytoplasmic tail. These phosphorylation events promote association between SHPS1 and multiple signaling proteins, including SHP-1, SHP-2, Grb2 and Shc via their SH2 domains (1-4). Recruitment of SHP-1 and SHP-2 results in SHPS1 dephosphorylation and suppression of tyrosine kinase signaling (1-3,5). The tyrosine kinase JAK2 associates with SHPS1 via its carboxy terminus and phosphorylates SHPS1 in response to extracellular stimuli (5). Research studies show that Src associates with and may phosphorylate SHPS1 in response to insulin (4). In macrophages, SHPS1 can form a complex with the Src pathway adaptor protein SKAP2, Fyn-binding protein FYB, and the tyrosine kinase PYK2 (6). The SHPS1 extracellular domain contains at least three IgG-like domains that interact with CD47, a ubiquitously expressed, integrin-associated protein that acts as a repressive cue in both immune and neuronal cells (7,8). The interaction between CD47 and SHPS1 on opposing cells can inhibit cellular migration (9), promote "tethering" between macrophages and target cells during engulfment (10), facilitate self versus non-self recognition (11), and maintain immune homeostasis (12). SHPS1 plays a critical role in modulating the immune response and inflammation, and may play a role in neuronal development (13,14). The interaction between SHPS1 and CD47 may be an exploitable target in cancer therapy (15-17).
  1. Kharitonenkov, A. et al. (1997) Nature 386, 181-6.
  2. Ochi, F. et al. (1997) Biochem Biophys Res Commun 239, 483-7.
  3. Takada, T. et al. (1998) J Biol Chem 273, 9234-42.
  4. Shen, X. et al. (2009) Mol Cell Proteomics 8, 1539-51.
  5. Stofega, M.R. et al. (2000) J Biol Chem 275, 28222-9.
  6. Timms, J.F. et al. (1999) Curr Biol 9, 927-30.
  7. Seiffert, M. et al. (1999) Blood 94, 3633-43.
  8. Vernon-Wilson, E.F. et al. (2000) Eur J Immunol 30, 2130-7.
  9. Motegi, S. et al. (2003) EMBO J 22, 2634-44.
  10. Tada, K. et al. (2003) J Immunol 171, 5718-26.
  11. van Beek, E.M. et al. (2005) J Immunol 175, 7781-7.
  12. Legrand, N. et al. (2011) Proc Natl Acad Sci U S A 108, 13224-9.
  13. Sarfati, M. et al. (2008) Curr Drug Targets 9, 842-50.
  14. Matozaki, T. et al. (2009) Trends Cell Biol 19, 72-80.
  15. Hara, K. et al. (2011) Cancer Res 71, 1229-34.
  16. Willingham, S.B. et al. (2012) Proc Natl Acad Sci U S A 109, 6662-7.
  17. Weiskopf, K. et al. (2013) Science 341, 88-91.

数据库链接

UniProt ID: P78324


Entrez-Gene Id: 140885


限制使用

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