Render Target: SSR
Render Timestamp: 2024-12-19T21:53:01.367Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-24 09:47:55.695
Product last modified at: 2024-09-20T07:06:43.322Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7
  1. Products /
  2. SignalStar™ Multiplex IHC Kits & Reagents /
  3. SIRPα/SHPS1 (D6I3M) & CO-0034-750 SignalStar™ Oligo-Antibody Pair

SIRPα/SHPS1 (D6I3M) & CO-0034-750 SignalStar™ Oligo-Antibody Pair #35593

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  • IHC

Order Information # 35593

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

    Product Information

    Product Usage Information

    Application Dilution
    SignalStar™ Leica Bond 1:50 - 1:200
    SignalStar™ Manual 1:50 - 1:200

    Storage

    SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

    Product Description

    SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

    SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

    Protocol

    Specificity / Sensitivity

    SIRPα/SHPS1 (D6I3M) Rabbit mAb (SignalStar™ Conjugate 0034) recognizes endogenous levels of total SHPS1 protein. This antibody recognizes both large and small isoforms of murine mSHPS1/SIRPα.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro413 of human SIRPα/SHPS1 protein.

    Background

    SHP-substrate 1 (SHPS1, SIRPα) is a single-pass membrane protein and member of both the immunoglobulin superfamily and the signal regulatory protein (SIRP) family. Following growth hormone stimulation or integrin binding, SHPS1 is phosphorylated at several tyrosine residues within its cytoplasmic tail. These phosphorylation events promote association between SHPS1 and multiple signaling proteins, including SHP-1, SHP-2, Grb2 and Shc via their SH2 domains (1-4). Recruitment of SHP-1 and SHP-2 results in SHPS1 dephosphorylation and suppression of tyrosine kinase signaling (1-3,5). The tyrosine kinase JAK2 associates with SHPS1 via its carboxy terminus and phosphorylates SHPS1 in response to extracellular stimuli (5). Research studies show that Src associates with and may phosphorylate SHPS1 in response to insulin (4). In macrophages, SHPS1 can form a complex with the Src pathway adaptor protein SKAP2, Fyn-binding protein FYB, and the tyrosine kinase PYK2 (6). The SHPS1 extracellular domain contains at least three IgG-like domains that interact with CD47, a ubiquitously expressed, integrin-associated protein that acts as a repressive cue in both immune and neuronal cells (7,8). The interaction between CD47 and SHPS1 on opposing cells can inhibit cellular migration (9), promote "tethering" between macrophages and target cells during engulfment (10), facilitate self versus non-self recognition (11), and maintain immune homeostasis (12). SHPS1 plays a critical role in modulating the immune response and inflammation, and may play a role in neuronal development (13,14). The interaction between SHPS1 and CD47 may be an exploitable target in cancer therapy (15-17).
    1. Kharitonenkov, A. et al. (1997) Nature 386, 181-6.
    2. Ochi, F. et al. (1997) Biochem Biophys Res Commun 239, 483-7.
    3. Takada, T. et al. (1998) J Biol Chem 273, 9234-42.
    4. Shen, X. et al. (2009) Mol Cell Proteomics 8, 1539-51.
    5. Stofega, M.R. et al. (2000) J Biol Chem 275, 28222-9.
    6. Timms, J.F. et al. (1999) Curr Biol 9, 927-30.
    7. Seiffert, M. et al. (1999) Blood 94, 3633-43.
    8. Vernon-Wilson, E.F. et al. (2000) Eur J Immunol 30, 2130-7.
    9. Motegi, S. et al. (2003) EMBO J 22, 2634-44.
    10. Tada, K. et al. (2003) J Immunol 171, 5718-26.
    11. van Beek, E.M. et al. (2005) J Immunol 175, 7781-7.
    12. Legrand, N. et al. (2011) Proc Natl Acad Sci U S A 108, 13224-9.
    13. Sarfati, M. et al. (2008) Curr Drug Targets 9, 842-50.
    14. Matozaki, T. et al. (2009) Trends Cell Biol 19, 72-80.
    15. Hara, K. et al. (2011) Cancer Res 71, 1229-34.
    16. Willingham, S.B. et al. (2012) Proc Natl Acad Sci U S A 109, 6662-7.
    17. Weiskopf, K. et al. (2013) Science 341, 88-91.

    Database Links

    UniProt ID: P78324


    Entrez-Gene Id: 140885


    限制使用

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    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    For Research Use Only. Not For Use In Diagnostic Procedures.
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