OX40 (E9U7O) & CO-0086-594 SignalStar™ Oligo-Antibody Pair #50642
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- IHC
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using OX40 (E9U7O) & CO-0086-488 SignalStar™ Oligo-Antibody Pair #91786 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Order Information # 50642
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
- Product Includes
- Related Products
| Product Includes | Volume | Reactivity | Isotype |
|---|---|---|---|
| OX40 (E9U7O) XP® Rabbit mAb (SignalStar™ Conjugate 0086) #42833 | 50 µl | H M | Rabbit IgG |
| Complementary Oligo (CO-0086-594) #28414 | 22 µl | H M |
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| SignalStar™ Leica Bond | 1:50 - 1:200 |
| SignalStar™ Manual | 1:50 - 1:200 |
Storage
SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.
Product Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
OX40 (E9U7O) XP® Rabbit mAb (SignalStar™ Conjugate 0086) recognizes endogenous levels of total OX40 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln264 of human OX40 protein.
Background
OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12).
- Croft, M. (2010) Annu Rev Immunol 28, 57-78.
- So, T. and Croft, M. (2012) Front Immunol 3, 133.
- Paterson, D.J. et al. (1987) Mol Immunol 24, 1281-90.
- Mallett, S. et al. (1990) EMBO J 9, 1063-8.
- Dürkop, H. et al. (1995) Br J Haematol 91, 927-31.
- Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62.
- Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82.
- So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8.
- So, T. and Croft, M. (2013) Front Immunol 4, 139.
- Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107.
- Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31.
- Linch, S.N. et al. (2015) Front Oncol 5, 34.
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