NeuN (D4G4O) & CO-0122-647 SignalStar™ Oligo-Antibody Pair #61599
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- IHC
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human Alzheimer’s brain using Tau (D1M9X) & CO-0109-488 SignalStar™ Oligo-Antibody Pair #90908 (cyan), Myelin Basic Protein (D8X4Q) & CO-0121-594 SignalStar™ Oligo-Antibody Pair #81137 (magenta), NeuN (D4G4O) & CO-0122-647 SignalStar™ Oligo-Antibody Pair #61599 (red), Iba1/AIF-1 (E4O4W) & CO-0100-594 SignalStar™ Oligo-Antibody Pair #71224 (green), VE-Cadherin (E6N7A) & CO-0149-647 SignalStar™ Oligo-Antibody Pair #95571 (white), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Order Information # 61599
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
- Product Includes
- Related Products
| Product Includes | Volume | Reactivity | Isotype |
|---|---|---|---|
| NeuN (D4G4O) XP® Rabbit mAb (SignalStar™ Conjugate 0122) #30737 | 50 µl | H M | Rabbit IgG |
| Complementary Oligo (CO-0122-647) #70508 | 22 µl |
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| SignalStar™ Leica Bond | 1:50 - 1:200 |
| SignalStar™ Manual | 1:50 - 1:200 |
Storage
SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.
Product Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
NeuN (D4G4O) XP® Rabbit mAb (SignalStar™ Conjugate 0122) recognizes endogenous levels of total NeuN protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NeuN protein.
Background
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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