Myeloperoxidase (E1E7I) & CO-0138-594 SignalStar™ Oligo-Antibody Pair #39503
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- IHC
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using Phospho-Histone H2A.X (Ser139) (D7T2V) & CO-0135-488 SignalStar™ Oligo-Antibody Pair #66478 (magenta), CD54/ICAM-1 (E3Q9N) & CO-0146-594 SignalStar™ Oligo-Antibody Pair #32147 (yellow), Myeloperoxidase (E1E7I) & CO-0138-647 SignalStar™ Oligo-Antibody Pair #53259 (red), Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (cyan), CD8α (D8A8Y) & CO-0004-488 SignalStar™ Oligo-Antibody Pair #45747 (green), Ki-67 (8D5) & CO-0014-594 SignalStar™ Oligo-Antibody Pair #35330 (white), and α-Smooth Muscle Actin (D4K9N) & CO-0024-647 SignalStar™ Oligo-Antibody Pair #63902 (orange). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Order Information # 39503
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
- Product Includes
- Related Products
| Product Includes | Volume | Reactivity | Isotype |
|---|---|---|---|
| Myeloperoxidase (E1E7I) XP® Rabbit mAb (SignalStar™ Conjugate 0138) #83214 | 50 µl | H | Rabbit IgG |
| Complementary Oligo (CO-0138-594) #75679 | 22 µl |
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| SignalStar™ Leica Bond | 1:50 - 1:200 |
| SignalStar™ Manual | 1:50 - 1:200 |
Storage
SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.
Product Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
Myeloperoxidase (E1E7I) XP® Rabbit mAb (SignalStar™ Conjugate 0138) recognizes endogenous levels of total myeloperoxidase protein. This antibody recognizes the full-length and heavy chain subunits of human myeloperoxidase protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro734 of human myeloperoxidase protein.
Background
Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1).
- Klebanoff, S.J. (2005) J Leukoc Biol 77, 598-625.
- Chang, K.S. et al. (1986) Blood 68, 1411-4.
- Yamada, M. et al. (1987) Arch Biochem Biophys 255, 147-55.
- Morishita, K. et al. (1987) J Biol Chem 262, 3844-51.
- Weil, S.C. et al. (1987) Proc Natl Acad Sci USA 84, 2057-61.
- Aratani, Y. et al. (2002) J Infect Dis 185, 1833-7.
限制使用
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