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Product last modified at: 2025-03-27T08:00:17.057Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7
  1. Products /
  2. SignalStar™ Multiplex IHC Kits & Reagents /
  3. IL-1β (3A6) Mouse mAb & CO-0175-488 SignalStar™ Oligo-Antibody Pair

IL-1β (3A6) Mouse mAb & CO-0175-488 SignalStar™ Oligo-Antibody Pair #48461

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  • IHC
Immunohistochemistry Image 1: IL-1β (3A6) Mouse mAb & CO-0175-488 SignalStar™ Oligo-Antibody Pair

SignalStarTM multiplex immunohistochemical analysis of paraffin-embedded human tonsil using IL-1β (3A6) Mouse mAb (488; green), CD86 (E2G8P) Rabbit mAb (594; cyan), MSH2 (D24B5) Rabbit mAb (647; red), CD68 (D4B9C) Rabbit mAb (647; white), CD8α (D8A8Y) Rabbit mAb (750; yellow), and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.

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Order Information # 48461

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
Product IncludesVolumeReactivityIsotype
IL-1β (3A6) Mouse mAb (SignalStar™ Conjugate 0175) #8149750 µlHMouse IgG1
Complementary Oligo (CO-0175-488) #4570830 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

IL-1β (3A6) Mouse mAb (SignalStar® Conjugate 0175) recognizes endogenous levels of total IL-1β protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.

Background

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

Database Links

UniProt ID: P01584


Entrez-Gene Id: 3553


限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
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