Render Target: SSR
Render Timestamp:
4/3/2025, 7:21:32 AM EDT
4/3/2025, 11:21:32 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:19:53.610
Product last modified at: 2025-03-06T09:00:17.400Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

CD68 (E3O7V) & CO-0096-488 SignalStar Oligo-Antibody Pair #29690

Filter:
  • IHC
Immunohistochemistry Image 1: CD68 (E3O7V) & CO-0096-488 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse thymus using CD20 (E3N7O) & CO-0134-488 SignalStar™ Oligo-Antibody Pair #18644 (red), TIM-3 (D3M9R) & CO-0094-594 SignalStar™ Oligo-Antibody Pair #55557 (yellow), and CD68 (E3O7V) & CO-0096-647 SignalStar™ Oligo-Antibody Pair #58388 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

Order Information # 29690

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
CD68 (E3O7V) Rabbit mAb (SignalStar™ Conjugate 0096) #4701350 µlMRabbit IgG
Complementary Oligo (CO-0096-488) #3457622 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

CD68 (E3O7V) Rabbit mAb (SignalStar™ Conjugate 0096) recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

Background

CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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