CD11c (D3V1E) & CO-0017-647 SignalStar™ Oligo-Antibody Pair #96411
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- IHC
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD11c (D3V1E) & CO-0017-647 SignalStar™ Oligo-Antibody Pair #96411 (magenta), CD163 (D6U1J) & CO-0022-488 SignalStar™ Oligo-Antibody Pair #35238 (green), PD-L1 (E1L3N®) & CO-0005-594 SignalStar™ Oligo-Antibody Pair #28249 (yellow), PD-1 (Intracellular Domain) (D4W2J) & CO-0008-647 SignalStar™ Oligo-Antibody Pair #56837 (red), CD68 (D4B9C) & CO-0007-750 SignalStar™ Oligo-Antibody Pair #91044 (cyan), HLA-DRA (E9R2Q) & CO-0023-488 SignalStar™ Oligo-Antibody Pair #92941 (pink), CD3ε (D7A6E™) & CO-0001-594 SignalStar™ Oligo-Antibody Pair #84634 (orange), Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (white), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Order Information # 96411
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
- Product Includes
- Related Products
| Product Includes | Volume | Reactivity | Isotype |
|---|---|---|---|
| CD11c (D3V1E) XP® Rabbit mAb (SignalStar™ Conjugate 0017) #94363 | 50 µl | H | Rabbit IgG |
| Complementary Oligo (CO-0017-647) #80527 | 22 µl |
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| SignalStar™ Leica Bond | 1:50 - 1:200 |
| SignalStar™ Manual | 1:50 - 1:200 |
Storage
SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.
Product Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
CD11c (D3V1E) XP® Rabbit mAb (SignalStar™ Conjugate 0017) recognizes endogenous levels of total CD11c protein. This antibody does not cross-react with CD11b protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CD11c protein.
Background
CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).
- Uotila, L.M. et al. (2013) J Biol Chem 288, 33494-9.
- Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
- Siegal, F.P. et al. (1999) Science 284, 1835-7.
- Racine, R. et al. (2008) J Immunol 181, 1375-85.
- Werfel, T. et al. (1991) J Immunol 147, 2423-7.
- Cabañas, C. et al. (1988) Hybridoma 7, 167-76.
- Kristensen, J.S. et al. (1987) Blood 70, 1063-8.
Pathways
Explore pathways related to this product.
限制使用
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