Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702
Filter:
- WB
Supporting Data
| REACTIVITY | Rab |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Rab-Rabbit
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
Product Usage Information
Recommended Antibody Dilutions:
1:1000-1:3000
Storage
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.
Species Reactivity:
Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Background
Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) as a secondary antibody will eliminate this problem.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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